Document Detail

IL-10 suppresses cell surface CD23/Fc epsilon RII expression, not by enhancing soluble CD23 release, but by reducing CD23 mRNA expression in human monocytes.
MedLine Citation:
PMID:  8946277     Owner:  NLM     Status:  MEDLINE    
To examine a possible involvement of interleukin-10 (IL-10) in CD23/Fc epsilon RII expression in human monocytes, effects of IL-10 on the cell surface CD23 expression, soluble CD23 (sCD23) release, and CD23 type b mRNA expression were investigated. IL-10 suppressed IL-4-induced surface CD23 expression on monocytes in a dose-dependent manner, and this effect was completely neutralized by anti-IL-10 antibody. The suppressive effect of IL-10 on surface CD23 expression was not due to enhancement of sCD23 release from the cell surface because no increase in sCD23 in culture supernatant was detected after incubation with IL-10. Instead, the effect of IL-10 seemed to be exerted at the transcriptional level since IL-4-induced expression of CD23 type b mRNA was significantly reduced when IL-10 was present. Although IL-4 induced surface CD23 expression on both monocytes and B cells, the suppressive effect of IL-10 was observed only on monocytes, which underscores different regulatory mechanisms for CD23 expression between the two cell types.
A Morinobu; S Kumagai; H Yanagida; H Ota; H Ishida; M Matsui; J Yodoi; K Nakao
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of clinical immunology     Volume:  16     ISSN:  0271-9142     ISO Abbreviation:  J. Clin. Immunol.     Publication Date:  1996 Nov 
Date Detail:
Created Date:  1997-02-19     Completed Date:  1997-02-19     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  8102137     Medline TA:  J Clin Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  326-33     Citation Subset:  IM    
Department of Medicine and Clinical Science, Kyoto University, Graduate School of Medicine, Japan.
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MeSH Terms
B-Lymphocytes / drug effects
Cell Membrane / drug effects
Cells, Cultured
Dose-Response Relationship, Drug
Enzyme-Linked Immunosorbent Assay
Flow Cytometry
Interleukin-10 / pharmacology*
Interleukin-4 / pharmacology
Monocytes / drug effects*
Polymerase Chain Reaction
RNA, Messenger / metabolism*
Receptors, IgE / genetics,  metabolism*
Reg. No./Substance:
0/RNA, Messenger; 0/Receptors, IgE; 130068-27-8/Interleukin-10; 207137-56-2/Interleukin-4

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