Document Detail


IL-10 inhibits lipopolysaccharide-induced murine B cell proliferation and cross-linking of surface antigen receptors or ligation of CD40 restores the response.
MedLine Citation:
PMID:  8871628     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of IL-10 on murine B cell proliferation in vitro were investigated. IL-10 inhibited LPS-induced B cell proliferation with an EC50 of approximately 500 pg/ml. IL-10-mediated inhibitory activity was not overtly associated with cytotoxicity or induction of apoptosis. The presence or the absence of T cells and mononuclear phagocytes did not affect the inhibitory activity of IL-10 on LPS-induced proliferation. LPS-stimulated, IL-10-exposed B cells progressed from G0 or from M to G1A of the cell cycle, but were inhibited from entry into subsequent phases. IL-10 had no discernible effect on B cell proliferation elicited with goat anti-mouse IgM plus IL-4. Moreover, cross-linking, but not mere ligation, of surface Ag receptors restored LPS-induced B cell proliferation in the presence of IL-10. The proliferative response to ligation of CD40 with anti-CD40 Abs was also not inhibited by IL-10, and as observed with goat anti-mouse IgM, the presence of such Abs in IL-10-containing B cell cultures allowed for the proliferative response to LPS. A variety of other Abs reactive with murine B cell surface markers were ineffective at modulating the response to IL-10. IL-1, IL-2, IL-4, IL-5, IL-6, IFN-gamma, and TGF-beta were also ineffective in this regard. These observations suggest that IL-10 has a role in the suppression of inappropriate B cell proliferation, i.e., proliferation by B cells that have not effectively interacted with relevant Ag or CD40 ligand.
Authors:
J F Marcelletti
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Publication Detail:
Type:  In Vitro; Journal Article    
Journal Detail:
Title:  Journal of immunology (Baltimore, Md. : 1950)     Volume:  157     ISSN:  0022-1767     ISO Abbreviation:  J. Immunol.     Publication Date:  1996 Oct 
Date Detail:
Created Date:  1996-12-17     Completed Date:  1996-12-17     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  2985117R     Medline TA:  J Immunol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3323-33     Citation Subset:  AIM; IM    
Affiliation:
LIDAK Pharmaceuticals, La Jolla, CA 92037, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies / pharmacology
Antigens, CD40 / metabolism*
B-Lymphocytes / cytology,  immunology*
CD40 Ligand
Cell Cycle
Cross-Linking Reagents
Cytokines / pharmacology
G1 Phase
Immunoglobulin M / pharmacology
Interleukin-10 / pharmacology*
Ligands
Lipopolysaccharides / pharmacology
Lymphocyte Activation
Membrane Glycoproteins / metabolism
Mice
Mice, Inbred A
Mice, Inbred BALB C
Receptors, Antigen, B-Cell / chemistry,  metabolism*
Spleen / cytology,  immunology
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigens, CD40; 0/Cross-Linking Reagents; 0/Cytokines; 0/Immunoglobulin M; 0/Ligands; 0/Lipopolysaccharides; 0/Membrane Glycoproteins; 0/Receptors, Antigen, B-Cell; 130068-27-8/Interleukin-10; 147205-72-9/CD40 Ligand

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