Document Detail

IGH and IGK gene rearrangements as PCR targets for pediatric Burkitt's lymphoma and mature B-ALL MRD analysis.
MedLine Citation:
PMID:  19668242     Owner:  NLM     Status:  MEDLINE    
We recently reported that minimal residual disease (MRD) and minimal disseminated disease (MDD), assessed by long-distance PCR (LD-PCR) for t(8;14), are negative prognostic factors in mature B-cell acute lymphoblastic leukemia (B-ALL) and in Burkitt's lymphoma (BL). However, t(8;14) is detectable in only about 70% of patients, thus preventing MRD studies by this approach in the remaining patients. At present, no molecular assays have been reported for MRD and MDD analysis in t(8;14)-negative patients. The aim of our study was to evaluate the characteristics of patient-specific immunoglobulin (Ig) gene rearrangements as RQ-PCR targets for MRD analysis, in order to extend MRD studies to those patients who are not eligible for the LD-PCR assay. The study was performed according to the guidelines of the European Study Group on MRD detection in ALL (ESG-MRD-ALL). Overall, 36 B-ALL and 19 BL cases were analyzed. Multiple PCR reactions were performed for each sample to identify heavy and kappa light-chain rearrangements. A total of 97 RQ-PCR targets (62 for B-ALL, 35 for BL) were analyzed for sensitivity. The rearrangement pattern identified was similar to that reported for normal peripheral blood lymphocytes. In 88% of the targets, a sensitivity of at least 10(-4) was achieved. In 87% of patients (84% of B-ALLs, 95% of BLs) at least one sensitive target was available. All PCR targets identified at diagnosis were preserved at relapse. Our results suggest that MDD and MRD can be successfully studied using a single sensitive Ig target in the great majority of B-ALL and BL cases. The combination of LD-PCR and Ig-based assays will allow MRD analysis in virtually all of the patients.
Federica Lovisa; Lara Mussolin; Lilia Corral; Marta Pillon; Giovanni Cazzaniga; Andrea Biondi; Angelo Rosolen
Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-08-10
Journal Detail:
Title:  Laboratory investigation; a journal of technical methods and pathology     Volume:  89     ISSN:  1530-0307     ISO Abbreviation:  Lab. Invest.     Publication Date:  2009 Oct 
Date Detail:
Created Date:  2009-09-28     Completed Date:  2009-10-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0376617     Medline TA:  Lab Invest     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1182-6     Citation Subset:  IM    
Clinica di Oncoematologia Pediatrica, Azienda Ospedaliera-Università di Padova, Padua, Italy.
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MeSH Terms
Burkitt Lymphoma / genetics*
Gene Rearrangement, B-Lymphocyte, Heavy Chain*
Gene Rearrangement, B-Lymphocyte, Light Chain*
Immunoglobulin Heavy Chains / genetics*
Immunoglobulin kappa-Chains / genetics*
Leukemia, B-Cell / genetics*
Neoplasm, Residual
Polymerase Chain Reaction
Reg. No./Substance:
0/Immunoglobulin Heavy Chains; 0/Immunoglobulin kappa-Chains

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