| IGF1-induced AKT phosphorylation and cell proliferation are suppressed with the increase in PTEN during luteinization in human granulosa cells. | |
| | |
MedLine Citation:
|
PMID: 19225041 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
|
Granulosa cells proliferate and then undergo differentiation; an inverse relationship between these processes is observed during terminal follicular growth. During terminal follicular growth and initial luteinization, there is a necessary transition of granulosa cells to a less proliferative and highly steroidogenic form in response to LH. Although the expression of several molecules has been reported to be up-regulated by LH, proliferation/differentiation transition is not fully understood. Here, we show that the expression of a tumor suppressor, phosphatase and tensin homologue deleted on chromosome 10 (PTEN) was induced with human chorionic gonadotropin (hCG) treatment in human luteinized granulosa cells. Pretreatment with hCG attenuated insulin-like growth factor (IGF)-1-induced phosphorylation of AKT and cell proliferation, not phosphorylation of ERK1/2. Moreover, suppression of hCG-induced PTEN expression with siRNA increased AKT phosphorylation and cell proliferation in response to IGF1. We also demonstrate that a PI3K inhibitor, LY294002, not a MEK inhibitor, PD98059, inhibited IGF1-induced cell proliferation. In conclusion, PTEN induced to express by hCG in luteinized granulosa cells that inactivates AKT, not ERK, and attenuates IGF1-induced cell proliferation. PTEN expression may be a trigger for proliferation/differentiation transition in human granulosa cells. |
| | |
Authors:
|
Maki Goto; Akira Iwase; Toko Harata; Sachiko Takigawa; Kyosuke Suzuki; Shuichi Manabe; Fumitaka Kikkawa |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-02-18 |
Journal Detail:
|
Title: Reproduction (Cambridge, England) Volume: 137 ISSN: 1741-7899 ISO Abbreviation: Reproduction Publication Date: 2009 May |
Date Detail:
|
Created Date: 2009-04-21 Completed Date: - Revised Date: - |
Medline Journal Info:
|
Nlm Unique ID: 100966036 Medline TA: Reproduction Country: England |
Other Details:
|
Languages: eng Pagination: 835-42 Citation Subset: IM |
Affiliation:
|
Departments of Obstetrics and Gynecology, Nagoya University Graduate School of Medicine, 65 Tsurumai-cho, Showa-ku, Nagoya 466-8550, Japan. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Different inhibitory effects in rat and human carboxylesterases.
Next Document: Rapid effects of LH on gene expression in the mural granulosa cells of mouse periovulatory follicles...