Document Detail

IGF binding protein-3 secreted by the prostate adenocarcinoma cells (PC-3): differential effect on PC-3 and normal prostate cell growth.
MedLine Citation:
PMID:  7693099     Owner:  NLM     Status:  MEDLINE    
Deregulation of growth in malignant cells has been suggested to be the result of increased secretion by these cells, of autocrine growth factors; alternatively, this deregulation has been assumed to be related to loss of sensitivity by malignant cells to secreted inhibitory molecules. The results of the present publication lend new support to both hypotheses. We recently showed that human prostate adenocarcinoma cells (PC-3 cells) secreted insulin-like growth factor binding proteins (IGFBP) of 45, 34 and 25 kDa. From medium conditioned by dense cultures of PC-3 cells, we have now purified two IGFBPs of M(r) 45 kDa and 34 kDa. The N-amino terminal sequences were determined, and it was shown that they are IGFBP-3. IGFBP-34 appeared to be a deglycosylated form of IGFBP-45. The two IGFBPs had more affinity for IGF-II than for IGF-I. IGFBP-45 and IGFBP-34 were growth-inhibitory factors of chick embryo fibroblasts (CEF): they totally inhibited DNA synthesis stimulated by serum in CEF. Our results point to a clear difference between the effects of these IGFBPs upon growth of normal prostate cells and malignant PC-3 cells. At a concentration of 150 ng/ml, they inhibited growth of normal prostate cells even in the presence of 1 microgram/ml insulin. This suggests that such inhibition was not simply the result of a decrease by the IGFBP of stimulation induced by serum IGF or IGF secreted by the cells. At a concentration of 150 ng/ml, IGFBP did not modify the growth of PC-3 cells. In contrast, it stimulated growth of PC-3 cells when added at a concentration lower than 50 ng/ml (about 1 nM). Our results thus provide new insight concerning the regulation of growth in PC-3 cells.
E Kaicer; C Blat; J Imbenotte; F Troalen; O Cussenot; F Calvo; L Harel
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Growth regulation     Volume:  3     ISSN:  0956-523X     ISO Abbreviation:  Growth Regul.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-12-10     Completed Date:  1993-12-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  9106990     Medline TA:  Growth Regul     Country:  SCOTLAND    
Other Details:
Languages:  eng     Pagination:  180-9     Citation Subset:  IM    
Institut de Recherches Scientifiques sur le Cancer, Villejuif, France.
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MeSH Terms
Adenocarcinoma / metabolism,  pathology*,  secretion*
Amino Acid Sequence
Blotting, Western
Carrier Proteins / pharmacology*,  secretion*
Cell Division / drug effects,  physiology
Cells, Cultured
Chick Embryo
Culture Media / pharmacology
DNA / metabolism
DNA, Neoplasm / metabolism
Dose-Response Relationship, Drug
Electrophoresis, Polyacrylamide Gel
Fibroblasts / cytology,  metabolism,  physiology
Insulin-Like Growth Factor Binding Proteins
Molecular Sequence Data
Molecular Weight
Prostate / cytology*,  physiology
Prostatic Neoplasms / metabolism,  pathology*,  secretion*
Tumor Cells, Cultured
Reg. No./Substance:
0/Carrier Proteins; 0/Culture Media; 0/DNA, Neoplasm; 0/Insulin-Like Growth Factor Binding Proteins; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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