| IGF-1 rescues human intervertebral annulus cells from in vitro stress-induced premature senescence. | |
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MedLine Citation:
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PMID: 19021034 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aging human intervertebral disc contains a sizeable population of senescent cells. Since senescent cells cannot divide, senescence reduces the disc's ability to generate new cells to replace existing ones lost to necrosis or apoptosis. The objectives of the present work were: (1) to develop a reliable in vitro model for stress-induced premature senescence in human annulus cells, and (2) to investigate the potential for insulin-like growth factor-1 (IGF-1) to prevent or ameliorate senescence in vitro. The developed experimental model employs a 2 h exposure to 50 microM hydrogen peroxide; immunocytochemical localization of senescence associated-beta-galactosidase at pH 6.0 was used as the marker for senescent cells, and the percentage of senescent cells quantified after 3 days of culture. Nine sets of annulus cells were obtained from eight human surgical disc specimens; cells were tested with 0, 50, 100 or 500 ng/ml IGF-1. Although 50 or 100 ng/ml IGF-1 did not significantly alter the percentage of senescent cells, a significant reduction was present following exposure to 500 ng/ml IGF-1 (control, 56.3% +/- 8.5 (9); mean +/- SEM, (n) vs. treated, 39.6% +/- 6.6 (9), p = 0.0009). These novel findings point to the value of continued research towards development of future biologic therapies designed to reduce cell senescence in degenerating human discs. |
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Authors:
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Helen E Gruber; Gretchen L Hoelscher; Jane A Ingram; Synthia Bethea; Edward N Hanley |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Growth factors (Chur, Switzerland) Volume: 26 ISSN: 0897-7194 ISO Abbreviation: Growth Factors Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2009-04-20 Completed Date: 2009-06-04 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9000468 Medline TA: Growth Factors Country: England |
Other Details:
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Languages: eng Pagination: 220-5 Citation Subset: IM |
Affiliation:
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Department of Orthopaedic Surgery, Carolinas Medical Center, PO Box 32861, Charlotte, NC 28232, USA. helen.gruber@carolinashealthcare.org |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Cell Aging
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drug effects* Cell Culture Techniques Cells, Cultured Humans Hydrogen Peroxide / pharmacology Immunohistochemistry Insulin-Like Growth Factor I / metabolism, pharmacology* Intervertebral Disk / cytology, drug effects*, metabolism Oxidants / pharmacology Oxidative Stress / drug effects* Spinal Diseases / pathology* beta-Galactosidase / metabolism |
| Chemical | |
Reg. No./Substance:
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0/Oxidants; 67763-96-6/Insulin-Like Growth Factor I; 7722-84-1/Hydrogen Peroxide; EC 3.2.1.23/beta-Galactosidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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