Document Detail

IFNgamma and TNFalpha synergistically induce neurite outgrowth on PC12 cells.
MedLine Citation:
PMID:  15763171     Owner:  NLM     Status:  MEDLINE    
PC12 cells are commonly used in the study of neuronal cells. It was reported that IFNgamma enhances neurite outgrowth of PC12 cells by NGF-stimuli. Accordingly, IFNgamma was examined to determine if it could solely produce neurite outgrowth. In addition, because the synergism between TNFalpha and IFNgamma is well-known, this study investigated whether or not a mixture of IFNgamma and TNFalpha might augment neurite outgrowth on PC12 cells. Finally, this study examined how an AG490 treatment, which was used to inhibit the IFNgamma signal in this study, affected the cytokine-mediated phenomenon. The results showed that the cytokines did not cause an increase in apoptosis in the PC12 cells and the serum-starved condition blocked the cytokine-mediated neurite outgrowth. Interestingly, AG490 enhanced this effect. In conclusion, it was shown that IFNgamma has the potential to form neurites, and TNFalpha can enhance this ability.
Sung Gook Cho; Seh Yoon Yi; Young Sook Yoo
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2005-01-24
Journal Detail:
Title:  Neuroscience letters     Volume:  378     ISSN:  0304-3940     ISO Abbreviation:  Neurosci. Lett.     Publication Date:  2005 Apr 
Date Detail:
Created Date:  2005-03-14     Completed Date:  2005-04-21     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7600130     Medline TA:  Neurosci Lett     Country:  Ireland    
Other Details:
Languages:  eng     Pagination:  49-54     Citation Subset:  IM    
Bioanalysis and Biotransformation Research Center, Korea Institute of Science and Technology, Seoul 130-650, South Korea.
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MeSH Terms
Cell Death / drug effects,  physiology
Cell Survival / drug effects,  physiology
Drug Synergism
Electrophoresis, Polyacrylamide Gel
Enzyme Inhibitors / pharmacology
Flow Cytometry
Interferon-gamma / pharmacology*
Neurites / drug effects*
PC12 Cells
Tumor Necrosis Factor-alpha / pharmacology*
Tyrphostins / pharmacology
Reg. No./Substance:
0/Enzyme Inhibitors; 0/Tumor Necrosis Factor-alpha; 0/Tyrphostins; 0/alpha-cyano-(3,4-dihydroxy)-N-benzylcinnamide; 82115-62-6/Interferon-gamma

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