| ID3 mediates X-ray-induced apoptosis of keratinocytes through the regulation of β-catenin. | |
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MedLine Citation:
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PMID: 21030215 Owner: NLM Status: In-Process |
Abstract/OtherAbstract:
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BACKGROUND: Ionizing radiation is used to treat many of cancers, however, it also produces unwanted side effect on normal tissues, such as radiodermatitis. We previously established an animal model for radiodermatitis, and found that X-ray irradiation induced the expression of ID3 in hairless mouse skin by cDNA microarray. OBJECTIVE: The aim of this study is to investigate the functional role of ID3 in X-ray irradiated keratinocytes. METHODS: Immunohistochemistry, RT-PCR and Western blot were performed to demonstrate the ID3 induction by X-ray irradiation. HaCaT keratinocytes were transduced with the recombinant adenovirus expressing HA-ID3, and then effects on apoptosis were analyzed. RESULTS: X-ray irradiation increased markedly the ID3 protein level in epidermis of mouse skin. X-ray irradiation also induced the expression of ID3 in HaCaT keratinocytes cultured in vitro, at both mRNA and protein levels. When ID3 was overexpressed by recombinant adenovirus, apoptosis of keratinocytes were induced even in the absence of X-ray irradiation. Furthermore, overexpression of ID3 sensitized X-ray-induced apoptosis. Interestingly, X-ray irradiation significantly reduced the endogenous β-catenin level, which was related with induction of apoptosis. Similarly, overexpression of ID3 led to remarkable reduction in β-catenin level. CONCLUSION: These results suggest that ID3 plays a role as an apoptosis inducer in response to X-ray irradiation via the regulation of endogenous β-catenin level. |
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Authors:
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Young-Sook Lee; Mohammad Lalmoddin Mollah; Kyung-Cheol Sohn; Ge Shi; Dae-Hun Kim; Ki-Hwan Kim; Moon-June Cho; Sooil Kim; Young Ho Lee; Chang Deok Kim; Jeung-Hoon Lee |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-09-24 |
Journal Detail:
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Title: Journal of dermatological science Volume: 60 ISSN: 1873-569X ISO Abbreviation: J. Dermatol. Sci. Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-11-29 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9011485 Medline TA: J Dermatol Sci Country: Netherlands |
Other Details:
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Languages: eng Pagination: 138-42 Citation Subset: IM |
Copyright Information:
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Copyright © 2010 Japanese Society for Investigative Dermatology. Published by Elsevier Ireland Ltd. All rights reserved. |
Affiliation:
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Department of Anatomy and Research Institute for Medical Sciences, Chungnam National University, Daejeon, Republic of Korea. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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