Document Detail


ICS 205-930 reduces 5-methoxytryptamine-induced short-circuit current in stripped pig jejunum.
MedLine Citation:
PMID:  7915189     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effect of ICS 205-930 on serotonin (5-hydroxytryptamine, 5-HT) and 5-methoxytryptamine (5-MeOT) induced short-circuit current (SCC) was studied in muscle-stripped pig jejunum in vitro. The selective 5-HT4 receptor agonist 5-MeOT and 5-HT both induced a concentration-dependent increase in SCC. The maximal efficacy of 5-HT was twice that of 5-MeOT. ICS 205-930 (Tropisetron) induced a small increase in SCC. Furthermore, ICS 205-930 reduced the 5-MeOT but not the 5-HT induced increase in SCC, except at the 100 microM ICS 205-930 concentration. Pretreatment with bumetanide reduced 5-HT, 5-MeOT, and ICS 205-930 induced peak increases in SCC by 64, 75, and 58%, respectively. Pretreatment with atropine reduced 5-HT, 5-MeOT, and ICS 205-930 induced peak increases in SCC by 38, 75, and 58%, respectively. Pretreatment with hexamethonium reduced 5-HT, 5-MeOT, and ICS 205-930 induced peak increases in SCC by 33, 50, and 36%, respectively. Pretreatment with tetrodotoxin reduced the peak increase in SCC elicited by 5-MeOT and ICS 205-930 by 41 and 50%, respectively. This study demonstrates involvement of a ICS 205-930, hexamethonium, atropine, bumetanide, and tetrodotoxin sensitive receptor in 5-MeOT induced SCC in muscle-stripped pig jejunum in vitro. These data suggest involvement of a neuronal 5-HT4 receptor subtype and acetylcholine in 5-HT elicited SCC and chloride secretion in the pig jejunum.
Authors:
M B Hansen
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Publication Detail:
Type:  In Vitro; Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Canadian journal of physiology and pharmacology     Volume:  72     ISSN:  0008-4212     ISO Abbreviation:  Can. J. Physiol. Pharmacol.     Publication Date:  1994 Mar 
Date Detail:
Created Date:  1994-09-29     Completed Date:  1994-09-29     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372712     Medline TA:  Can J Physiol Pharmacol     Country:  CANADA    
Other Details:
Languages:  eng     Pagination:  227-32     Citation Subset:  IM    
Affiliation:
Department of Anatomy and Physiology, Royal Veterinary and Agricultural University, Frederiksberg C, Denmark.
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MeSH Terms
Descriptor/Qualifier:
5-Methoxytryptamine / antagonists & inhibitors*,  pharmacology*
Animals
Atropine / pharmacology
Bumetanide / pharmacology
Drug Interactions
Electrophysiology
Female
Hexamethonium
Hexamethonium Compounds / pharmacology
Indoles / pharmacology*
Jejunum / drug effects*,  physiology*,  ultrastructure
Kinetics
Receptors, Serotonin / classification,  drug effects,  physiology
Serotonin / pharmacology
Serotonin Antagonists / pharmacology*
Swine
Tetrodotoxin / pharmacology
Theophylline / pharmacology
Chemical
Reg. No./Substance:
0/Hexamethonium Compounds; 0/Indoles; 0/Receptors, Serotonin; 0/Serotonin Antagonists; 28395-03-1/Bumetanide; 4368-28-9/Tetrodotoxin; 50-67-9/Serotonin; 51-55-8/Atropine; 58-55-9/Theophylline; 60-26-4/Hexamethonium; 608-07-1/5-Methoxytryptamine; 89565-68-4/tropisetron

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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