Document Detail

Hypoxic preconditioning protects rat hearts against ischaemia-reperfusion injury: role of erythropoietin on progenitor cell mobilization.
MedLine Citation:
PMID:  18845609     Owner:  NLM     Status:  MEDLINE    
Preconditioning, such as by brief hypoxic exposure, has been shown to protect hearts against severe ischaemia. Here we hypothesized that hypoxic preconditioning (HPC) protects injured hearts by mobilizing the circulating progenitor cells. Ischaemia-reperfusion (IR) injury was induced by left coronary ligation and release in rats kept in room air or preconditioned with 10% oxygen for 6 weeks. To study the role of erythropoietin (EPO), another HPC + IR group was given an EPO receptor (EPOR) antibody via a subcutaneous mini-osmotic pump 3 weeks before IR induction. HPC alone gradually increased haematocrit, cardiac and plasma EPO, and cardiac vascular endothelial growth factor (VEGF) only in the first two weeks. HPC improved heart contractility, reduced ischaemic injury, and maintained EPO and EPOR levels in the infarct tissues of IR hearts, but had no significant effect on VEGF. Interestingly, the number of CD34(+)CXCR4(+) cells in the peripheral blood and their expression in HPC-treated hearts was higher than in control. Preconditioning up-regulated cardiac expression of stromal derived factor-1 (SDF-1) and prevented its IR-induced reduction. The EPOR antibody abolished HPC-mediated functional recovery, and reduced SDF-1, CXCR4 and CD34 expression in IR hearts, as well as the number of CD34(+)CXCR4(+) cells in blood. The specificity of neutralizing antibody was confirmed in an H9c2 culture system. In conclusion, exposure of rats to moderate hypoxia leads to an increase in progenitor cells in the heart and circulation. This effect is dependent on EPO, which induces cell homing by increased SDF-1/CXCR4 and reduces the heart susceptibly to IR injury.
Jih-Shyong Lin; Yih-Sharng Chen; Han-Sun Chiang; Ming-Chieh Ma
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Publication Detail:
Type:  Journal Article     Date:  2008-10-09
Journal Detail:
Title:  The Journal of physiology     Volume:  586     ISSN:  1469-7793     ISO Abbreviation:  J. Physiol. (Lond.)     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2008-12-01     Completed Date:  2009-02-23     Revised Date:  2013-06-05    
Medline Journal Info:
Nlm Unique ID:  0266262     Medline TA:  J Physiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  5757-69     Citation Subset:  IM    
School of Medicine, Fu Jen Catholic University, 510 Chungcheng Road, Hsinchuang 242, Taiwan.
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MeSH Terms
Anoxia / blood,  metabolism,  physiopathology*
Antibodies / immunology,  pharmacology
Antigens, CD34 / genetics,  metabolism
Chemokine CXCL12 / metabolism
Creatine Kinase, MB Form / metabolism
Erythropoietin / blood,  pharmacology,  physiology*
Gene Expression / drug effects
Heart / drug effects,  physiopathology
Ischemic Preconditioning, Myocardial / methods*
Models, Biological
Myocardium / cytology,  metabolism
Myocytes, Cardiac / cytology,  drug effects,  metabolism
Rats, Wistar
Receptors, CXCR4 / genetics,  metabolism
Receptors, Erythropoietin / antagonists & inhibitors,  immunology,  metabolism
Reperfusion Injury / metabolism,  physiopathology*,  prevention & control
Reverse Transcriptase Polymerase Chain Reaction
Stem Cells / cytology,  drug effects,  metabolism
Vascular Endothelial Growth Factor A / metabolism
Ventricular Function, Left
Reg. No./Substance:
0/Antibodies; 0/Antigens, CD34; 0/Chemokine CXCL12; 0/Cxcr4 protein, rat; 0/Receptors, CXCR4; 0/Receptors, Erythropoietin; 0/Vascular Endothelial Growth Factor A; 0/vascular endothelial growth factor A, rat; 11096-26-7/Erythropoietin; EC Kinase, MB Form

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