| Hypoxic effects on glutamate uptake in cultured glial cells. | |
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MedLine Citation:
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PMID: 8098565 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hypoxic effects on glutamate uptake and ATP content in glial cells were investigated by using cultured C6 glioma cells. Mild regressive changes were found depending on the duration of the hypoxic insult, but necrosis or detachment of the cells from the substratum was rarely observed. Glutamate uptake was relatively well preserved after a short hypoxic insult, while a marked decrease in glutamate uptake was observed after hypoxia of long duration. The uptake of sucrose was reduced in a similar pattern to glutamate uptake. Hypoxic insult resulted in a significant reduction of the ATP content in glial cells. Therefore, the decrease in glutamate uptake by glial cells under hypoxia is likely to be due to ATP dependency, and not to the failure of a specific glutamate uptake system, but the failure of a general uptake of the glial cells owing to the energy-dependent membrane dysfunction by ATP depletion. These findings suggest that there are phased changes of astrocytic functions in a hypoxic condition, a preservative phase in the initial stages and then a dysfunctional phase in the later stages of hypoxia. |
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Authors:
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M Ohashi; S Amano; F Hazama; J Handa |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Acta pathologica japonica Volume: 43 ISSN: 0001-6632 ISO Abbreviation: Acta Pathol. Jpn. Publication Date: 1993 Apr |
Date Detail:
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Created Date: 1993-06-16 Completed Date: 1993-06-16 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0372637 Medline TA: Acta Pathol Jpn Country: AUSTRALIA |
Other Details:
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Languages: eng Pagination: 154-9 Citation Subset: IM |
Affiliation:
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Department of Neurosurgery, Shiga University of Medical Science, Otsu, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Adenosine Triphosphate
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analysis* Animals Cell Hypoxia Glioma Glutamates / pharmacokinetics* Glutamic Acid Neuroglia / chemistry, drug effects, metabolism* Tumor Cells, Cultured |
| Chemical | |
Reg. No./Substance:
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0/Glutamates; 56-65-5/Adenosine Triphosphate; 56-86-0/Glutamic Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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