| Hypoxia upregulates PGI-synthase and increases PGI₂ release in human vascular cells exposed to inflammatory stimuli. | |
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MedLine Citation:
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PMID: 21296955 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hypoxia affects vascular function and cell metabolism, survival, growth, and motility; these processes are partially regulated by prostanoids. We analyzed the effect of hypoxia and inflammation on key enzymes involved in prostanoid biosynthesis in human vascular cells. In human vascular smooth muscle cells (VSMC), hypoxia and interleukin (IL)-1β synergistically increased prostaglandin (PG)I₂ but not PGE₂ release, thereby increasing the PGI₂/PGE₂ ratio. Concomitantly, these stimuli upregulated cyclooxygenase-2 (COX-2) expression (mRNA and protein) and COX activity. Interestingly, hypoxia enhanced PGI-synthase (PGIS) expression and activity in VSMC and human endothelial cells. Hypoxia did not significantly modify the inducible microsomal-PGE-synthase (mPGES)-1. Hypoxia-inducible factor (HIF)-1α-silencing abrogated hypoxia-induced PGIS upregulation. PGIS transcriptional activity was enhanced by hypoxia; however, the minimal PGIS promoter responsive to hypoxia (-131 bp) did not contain any putative hypoxia response element (HRE), suggesting that HIF-1 does not directly drive PGIS transcription. Serial deletion and site-directed mutagenesis studies suggested several transcription factors participate cooperatively. Plasma levels of the stable metabolite of PGI₂ and PGIS expression in several tissues were also upregulated in mice exposed to hypoxia. These data suggest that PGIS upregulation is part of the adaptive response of vascular cells to hypoxic stress and could play a role in counteracting the deleterious effect of inflammatory stimuli. |
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Authors:
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Mercedes Camacho; Cristina Rodríguez; Anna Guadall; Sonia Alcolea; Mar Orriols; José-Román Escudero; José Martínez-González; Luis Vila |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2011-02-04 |
Journal Detail:
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Title: Journal of lipid research Volume: 52 ISSN: 0022-2275 ISO Abbreviation: J. Lipid Res. Publication Date: 2011 Apr |
Date Detail:
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Created Date: 2011-03-24 Completed Date: 2011-06-23 Revised Date: 2013-05-26 |
Medline Journal Info:
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Nlm Unique ID: 0376606 Medline TA: J Lipid Res Country: United States |
Other Details:
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Languages: eng Pagination: 720-31 Citation Subset: IM |
Affiliation:
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Angiology, Vascular Biology, and Inflammation Laboratory, Institute of Biomedical Research (IIB-Sant Pau), Barcelona, Spain. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blotting, Western Cell Hypoxia / genetics, physiology* Cells, Cultured Culture Media, Conditioned / pharmacology Cyclooxygenase 2 / metabolism Dinoprostone / metabolism Endothelial Cells / drug effects, enzymology, metabolism Epoprostenol / metabolism* Humans Interleukin-1beta / pharmacology* Male Mice Mice, Inbred C57BL Muscle, Smooth, Vascular / cytology* Myocytes, Smooth Muscle / drug effects, enzymology*, metabolism* Polymerase Chain Reaction Promoter Regions, Genetic / genetics Prostaglandin-Endoperoxide Synthases / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Conditioned; 0/Interleukin-1beta; 35121-78-9/Epoprostenol; 363-24-6/Dinoprostone; EC 1.14.99.1/Cyclooxygenase 2; EC 1.14.99.1/Prostaglandin-Endoperoxide Synthases |
| Comments/Corrections | |
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