| Hypoxia-ischemia induces thioredoxin expression and nitrotyrosine formation in new-born rat brain. | |
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MedLine Citation:
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PMID: 12688505 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Thioredoxin (TRX) is a 13 kDa protein with antioxidant effect and redox regulating functions. Peroxynitrite is a strong oxidizing and nitrating agent which can react with all classes of biomolecules. In the present study, we focused on the association between TRX and nitrotyrosine, which served as a marker of peroxynitrite formation, in the neonatal hypoxia-ischemia (HI) rat brain. At 4-16 h after HI, the immunoreactivity for TRX was diminished in the injured region in the cortex and striatum, whereas nitrotyrosine immunoreactivity was enhanced. In contrast, around the injured region, TRX immunoreactivity was enhanced in survival neurons at 4-24 h after HI, while the immunoreactivity for nitrotyrosine was mostly not detected. Northern blot analysis showed increased TRX mRNA induction in the cerebral hemisphere ipsilateral to the carotid ligation from 4-24 h after HI but not in the contralateral hypoxic hemisphere. These findings suggest that production of peroxynitrite is involved in HI brain injury, and that induced TRX plays a neuroprotective role against oxidative stress resulting from HI. |
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Authors:
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Itaro Hattori; Yasushi Takagi; Kazuhiko Nozaki; Norihiko Kondo; Jie Bai; Hajime Nakamura; Nobuo Hashimoto; Junji Yodoi |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Redox report : communications in free radical research Volume: 7 ISSN: 1351-0002 ISO Abbreviation: Redox Rep. Publication Date: 2002 |
Date Detail:
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Created Date: 2003-04-11 Completed Date: 2003-08-25 Revised Date: 2007-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9511366 Medline TA: Redox Rep Country: England |
Other Details:
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Languages: eng Pagination: 256-9 Citation Subset: IM |
Affiliation:
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Department of Neurosurgery, Kyoto University Graduate School of Medicine, Sakyo, Kyoto, Japan. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Animals, Newborn Anoxia* Blotting, Northern Brain / metabolism, pathology* Models, Anatomic Oxidants / pharmacology Peroxynitrous Acid / pharmacology RNA, Messenger / metabolism Rats Rats, Sprague-Dawley Reperfusion Injury* Thioredoxins / biosynthesis* Time Factors Tyrosine / analogs & derivatives*, chemistry*, metabolism |
| Chemical | |
Reg. No./Substance:
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0/Oxidants; 0/RNA, Messenger; 14691-52-2/Peroxynitrous Acid; 3604-79-3/3-nitrotyrosine; 52500-60-4/Thioredoxins; 55520-40-6/Tyrosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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