Document Detail

Hypoxia-ischemia induces thioredoxin expression and nitrotyrosine formation in new-born rat brain.
MedLine Citation:
PMID:  12688505     Owner:  NLM     Status:  MEDLINE    
Thioredoxin (TRX) is a 13 kDa protein with antioxidant effect and redox regulating functions. Peroxynitrite is a strong oxidizing and nitrating agent which can react with all classes of biomolecules. In the present study, we focused on the association between TRX and nitrotyrosine, which served as a marker of peroxynitrite formation, in the neonatal hypoxia-ischemia (HI) rat brain. At 4-16 h after HI, the immunoreactivity for TRX was diminished in the injured region in the cortex and striatum, whereas nitrotyrosine immunoreactivity was enhanced. In contrast, around the injured region, TRX immunoreactivity was enhanced in survival neurons at 4-24 h after HI, while the immunoreactivity for nitrotyrosine was mostly not detected. Northern blot analysis showed increased TRX mRNA induction in the cerebral hemisphere ipsilateral to the carotid ligation from 4-24 h after HI but not in the contralateral hypoxic hemisphere. These findings suggest that production of peroxynitrite is involved in HI brain injury, and that induced TRX plays a neuroprotective role against oxidative stress resulting from HI.
Itaro Hattori; Yasushi Takagi; Kazuhiko Nozaki; Norihiko Kondo; Jie Bai; Hajime Nakamura; Nobuo Hashimoto; Junji Yodoi
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Redox report : communications in free radical research     Volume:  7     ISSN:  1351-0002     ISO Abbreviation:  Redox Rep.     Publication Date:  2002  
Date Detail:
Created Date:  2003-04-11     Completed Date:  2003-08-25     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  9511366     Medline TA:  Redox Rep     Country:  England    
Other Details:
Languages:  eng     Pagination:  256-9     Citation Subset:  IM    
Department of Neurosurgery, Kyoto University Graduate School of Medicine, Sakyo, Kyoto, Japan.
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MeSH Terms
Animals, Newborn
Blotting, Northern
Brain / metabolism,  pathology*
Models, Anatomic
Oxidants / pharmacology
Peroxynitrous Acid / pharmacology
RNA, Messenger / metabolism
Rats, Sprague-Dawley
Reperfusion Injury*
Thioredoxins / biosynthesis*
Time Factors
Tyrosine / analogs & derivatives*,  chemistry*,  metabolism
Reg. No./Substance:
0/Oxidants; 0/RNA, Messenger; 14691-52-2/Peroxynitrous Acid; 3604-79-3/3-nitrotyrosine; 52500-60-4/Thioredoxins; 55520-40-6/Tyrosine

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