Document Detail


Hypoxia inducible factor-1 alpha and vascular endothelial growth factor in biopsies of small cell lung carcinoma.
MedLine Citation:
PMID:  19707816     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Neoangiogenesis has been documented in small cell lung carcinoma (SCLC). In addition, antiangiogenic therapies are being tested in clinical trials that involve SCLC. However, study of the underlying mechanisms has been performed almost exclusively in cell lines. In the current study, we immunostained 30 biopsy samples of SCLC with antibodies to hypoxia inducible factor-1 alpha (HIF-1 alpha), vascular endothelial growth factor (VEGF), vascular endothelial growth factor-receptor 1 (VEGF-R1/flt-1) and vascular endothelial growth factor-receptor 2 (VEGF-R1/flk-1). The immunoreactivity was analyzed using a bivariate Spearman correlation test and linear regression analysis. We found significant correlation between HIF-1 alpha nuclear staining and VEGF staining. Moreover HIF-1 alpha+/VEGF+ cases were associated with poor survival. We also found a positive correlation between VEGF and VEGF-R2 expression. We suggest that a HIF-1 alpha/VEGF angiogenic pathway may exist in vivo in SCLC, similar to that in non-SCLC. Our data also suggest a potential VEGF/VEGFR-2 autocrine pathway in SCLC. The inclusion of novel inhibitors to HIF-1 alpha and other factors may optimize antiangiogenic interventions in SCLC.
Authors:
M Ioannou; R Papamichali; E Kouvaras; I Mylonis; D Vageli; T Kerenidou; S Barbanis; A Daponte; G Simos; K Gourgoulianis; G K Koukoulis
Publication Detail:
Type:  Journal Article     Date:  2009-08-26
Journal Detail:
Title:  Lung     Volume:  187     ISSN:  1432-1750     ISO Abbreviation:  Lung     Publication Date:    2009 Sep-Oct
Date Detail:
Created Date:  2009-09-16     Completed Date:  2009-12-01     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7701875     Medline TA:  Lung     Country:  United States    
Other Details:
Languages:  eng     Pagination:  321-9     Citation Subset:  IM    
Affiliation:
Department of Pathology, Medical School, University of Thessaly, Mezourlo, Larissa, 41110, Greece.
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MeSH Terms
Descriptor/Qualifier:
Aged
Aged, 80 and over
Biopsy
Female
Humans
Hypoxia-Inducible Factor 1, alpha Subunit / analysis*
Immunohistochemistry
Kaplan-Meiers Estimate
Linear Models
Lung Neoplasms / blood supply,  chemistry*,  mortality,  pathology,  therapy
Male
Middle Aged
Neoplasm Staging
Proportional Hazards Models
Risk Assessment
Small Cell Lung Carcinoma / blood supply,  chemistry*,  mortality,  pathology,  therapy
Time Factors
Treatment Outcome
Vascular Endothelial Growth Factor A / analysis*
Vascular Endothelial Growth Factor Receptor-1 / analysis
Vascular Endothelial Growth Factor Receptor-2 / analysis
Chemical
Reg. No./Substance:
0/HIF1A protein, human; 0/Hypoxia-Inducible Factor 1, alpha Subunit; 0/VEGFA protein, human; 0/Vascular Endothelial Growth Factor A; EC 2.7.10.1/FLT1 protein, human; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-1; EC 2.7.10.1/Vascular Endothelial Growth Factor Receptor-2

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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