Document Detail

Hypoxia increases breast cancer cell-induced lymphatic endothelial cell migration.
MedLine Citation:
PMID:  18392137     Owner:  NLM     Status:  MEDLINE    
Because tumors are characterized by hypoxic environments, we used a novel in vitro noninvasive magnetic resonance imaging assay to examine the influence of invasive MDA-MB-231 breast cancer cells on the invasion and migration of human dermal lymphatic microvascular endothelial cells (HMVEC-dLy) under normoxic and hypoxic conditions. Nonmalignant immortalized MCF-12A human mammary epithelial cells instead of cancer cells or chambers with HMVEC-dLy alone were used as controls for comparison. HMVEC-dLy cells were labeled with a T(2) contrast agent (Feridex), and their invasion and migration through extracellular matrix under normoxic and hypoxic conditions were monitored using magnetic resonance imaging. A significant increase in the invasion and migration of HMVEC-dLy cells was detected in the presence of cancer cells, which further increased significantly under hypoxic conditions. HMVEC-dLy cells formed interconnecting strands extending toward the cancer cells under normoxic but not under hypoxic conditions. Following reoxygenation, these interconnecting strands, extending from HMVEC-dLy cells toward the cancer cells, were observed. These data demonstrate the importance of hypoxia in lymphatic endothelial cell invasion and migration through extracellular matrix in the presence of cancer cells.
Maria Mikhaylova; Noriko Mori; Flonné B Wildes; Piotr Walczak; Barjor Gimi; Zaver M Bhujwalla
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Neoplasia (New York, N.Y.)     Volume:  10     ISSN:  1476-5586     ISO Abbreviation:  Neoplasia     Publication Date:  2008 Apr 
Date Detail:
Created Date:  2008-04-08     Completed Date:  2008-05-22     Revised Date:  2013-08-12    
Medline Journal Info:
Nlm Unique ID:  100886622     Medline TA:  Neoplasia     Country:  Canada    
Other Details:
Languages:  eng     Pagination:  380-9     Citation Subset:  IM    
JHU ICMIC Program, The Russell H. Morgan Department of Radiology and Radiological Science, The Johns Hopkins University School of Medicine, Baltimore, MD 21205, USA.
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MeSH Terms
Anoxia / complications*
Breast Neoplasms / metabolism,  pathology*
Cell Communication
Cell Movement*
Cell Proliferation
Cells, Cultured
Drug Combinations
Endothelial Cells / metabolism,  pathology*
Enzyme-Linked Immunosorbent Assay
Ferric Compounds / chemistry
Magnetic Resonance Imaging
Neoplasm Invasiveness
Oxygen / metabolism
Skin / metabolism,  pathology*
Vascular Endothelial Growth Factor C
Grant Support
P50 CA103175/CA/NCI NIH HHS; P50 CA103175/CA/NCI NIH HHS; R01 CA082337/CA/NCI NIH HHS; R01 CA82337/CA/NCI NIH HHS
Reg. No./Substance:
0/Drug Combinations; 0/Ferric Compounds; 0/Laminin; 0/Proteoglycans; 0/Vascular Endothelial Growth Factor C; 119978-18-6/matrigel; 1309-37-1/ferric oxide; 7782-44-7/Oxygen; 9007-34-5/Collagen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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