Document Detail

Hypoxia enhances the relaxing influence of perivascular adipose tissue in isolated mice aorta.
MedLine Citation:
PMID:  20553914     Owner:  NLM     Status:  MEDLINE    
Adipose tissue releases an "adipocyte-derived relaxing factor" (ADRF) lowering tone of isolated arteries. The potential influence of hypoxia on the vasorelaxing properties of adipose tissue was investigated. Aortas from male Swiss mice with or without adherent adipose tissue were mounted in a wire myograph for isometric tension recording. Hypoxia (bubbling with 95% N(2), 5% CO(2)) relaxed precontracted (norephinephrine, 5 microM) aorta with adipose tissue while only a minimal vasorelaxing effect was observed in arteries without adipose tissue. This effect was also seen after precontraction with prostaglandin F(2alpha) (30 microM) or U-46619 (10 nM). Precontraction with 60 or 120 mM K(+), incubation with tetraethylammoniumchloride (3 mM) or glibenclamide (30 microM) significantly impaired the hypoxic response. Glibenclamide (30 microM) enhanced the vasorelaxing effect of NaHS (except at high concentrations of NaHS). Lactate (10 nM to 1 mM) had no effect on preparations with or without adipose tissue. 8-(p-sulfophenyl)theophylline (0.1 mM), zinc protoporphyrin IX (10 microM), 1 H-[1, 2, 4]oxadiazolo[4,3-A]quinoxalin-1-one (10 microM) and removal of the endothelium did not influence the hypoxic relaxation. Our findings indicate that hypoxia has a relaxing influence on mice aorta that is dependent on the presence of adherent adipose tissue. This relaxation is partly mediated by opening K(ATP) channels and independent of the endothelium and soluble guanylyl cyclase. Neither lactate, adenosine, CO nor H(2)S seems to be involved in this hypoxic response. However, the involvement of the as yet unidentified "adipocyte-derived relaxing factor" (ADRF) cannot be excluded.
Nele Maenhaut; Charlotte Boydens; Johan Van de Voorde
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-06-08
Journal Detail:
Title:  European journal of pharmacology     Volume:  641     ISSN:  1879-0712     ISO Abbreviation:  Eur. J. Pharmacol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-07-15     Completed Date:  2011-01-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  1254354     Medline TA:  Eur J Pharmacol     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  207-12     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2010 Elsevier B.V. All rights reserved.
Department of Pharmacology, Ghent University, De Pintelaan 185, Ghent, Belgium.
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MeSH Terms
15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid / pharmacology
Adenosine / pharmacology
Adipose Tissue / metabolism*
Aorta, Thoracic / drug effects,  physiology*
Arteries / drug effects
Cell Hypoxia / physiology*
Dinoprost / pharmacology
Glyburide / pharmacology
Guanylate Cyclase / pharmacology
Norepinephrine / pharmacology
Potassium / pharmacology
Prostaglandins / pharmacology
Receptors, Cytoplasmic and Nuclear / pharmacology
Sulfides / pharmacology
Tetraethylammonium / pharmacology
Theophylline / analogs & derivatives,  pharmacology
Vasodilation / physiology*
Reg. No./Substance:
0/Prostaglandins; 0/Receptors, Cytoplasmic and Nuclear; 0/Sulfides; 10238-21-8/Glyburide; 16721-80-5/sodium bisulfide; 51-41-2/Norepinephrine; 551-11-1/Dinoprost; 58-55-9/Theophylline; 58-61-7/Adenosine; 66-40-0/Tetraethylammonium; 7440-09-7/Potassium; 76898-47-0/15-Hydroxy-11 alpha,9 alpha-(epoxymethano)prosta-5,13-dienoic Acid; 80206-91-3/8-(4-sulfophenyl)theophylline; EC Cyclase; EC guanylyl cyclase

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