Document Detail

Hypoxia differently modulates gene expression of inositol 1,4,5-trisphosphate receptors in mouse kidney and HEK 293 cell line.
MedLine Citation:
PMID:  19120137     Owner:  NLM     Status:  MEDLINE    
Hypoxia is a state of insufficient oxygen supply of the tissue or cell. Kidney tissue is highly sensitive to oxygen deprivation and easily develops renal ischemic injury. Calcium transporters very sensitively react to oxygen deficiency. We investigated whether hypoxia affects the gene expression of intracellular calcium transporters in the intact kidney, and we compared the response to that of HEK 293 cells. Our results showed that, while in mouse kidney tissue hypoxia elevates mRNA for inositol 1,4,5-trisphosphate receptors (IP3R) type 1 (IP3R1) and ryanodine receptors (RyR) type 2 (RyR2), in culture of HEK 293 cells the gene expression of all IP3Rs decreased without affecting viability of the cells. RyR2 mRNA in HEK 293 cells was not significantly changed, but RyR1 gene expression was significantly increased by hypoxia. The different response of kidney tissue and HEK 293 cells to hypoxia could be due to unequal differentiation state of the cells in intact tissue and cultured embryonic cell line. The physiological relevance of this observation remains to be determined.
Dana Jurkovicova; Barbora Sedlakova; Lubica Lacinova; Juraj Kopacek; Zdena Sulova; Jan Sedlak; Olga Krizanova
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Annals of the New York Academy of Sciences     Volume:  1148     ISSN:  1749-6632     ISO Abbreviation:  Ann. N. Y. Acad. Sci.     Publication Date:  2008 Dec 
Date Detail:
Created Date:  2009-01-05     Completed Date:  2009-01-23     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7506858     Medline TA:  Ann N Y Acad Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  421-7     Citation Subset:  IM    
Institute of Molecular Physiology and Genetics, Centre of Excellence for Cardiovascular Research SAS, Bratislava, Slovakia.
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MeSH Terms
Apoptosis / physiology
Cell Line
Gene Expression Regulation*
Inositol 1,4,5-Trisphosphate Receptors* / genetics,  metabolism
Kidney* / cytology,  metabolism
Kidney Failure, Acute / physiopathology
Mice, Inbred C57BL
Protein Isoforms* / genetics,  metabolism
Ryanodine Receptor Calcium Release Channel / genetics,  metabolism
Reg. No./Substance:
0/Inositol 1,4,5-Trisphosphate Receptors; 0/Protein Isoforms; 0/Ryanodine Receptor Calcium Release Channel

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