| Hypoxia attenuates effector-target cell interaction in the airway and pulmonary vascular compartment. | |
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MedLine Citation:
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PMID: 17892511 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Leucocyte infiltration is known to play an important role in hypoxia-induced tissue damage. However, little information is available about hypoxia and interaction of effector (neutrophils) with target cells (alveolar epithelial cells, AEC; rat pulmonary artery endothelial cells, RPAEC). The goal of this study was to elucidate hypoxia-induced changes of effector-target cell interaction. AEC and RPAEC were exposed to 5% oxygen for 2-6 h. Intercellular adhesion molecule-1 (ICAM-1) expression was determined and cell adherence as well as cytotoxicity assays were performed. Nitric oxide and heat shock protein 70 (HSP70) production was assessed in target cells. Under hypoxic conditions enhanced ICAM-1 production was found in both cell types. This resulted in an increase of adherent neutrophils to AEC and RPAEC. The death rate of hypoxia-exposed target cells decreased significantly in comparison to control cells. Nitric oxide (NO) concentration was enhanced, as was production of HSP70 in AEC. Blocking NO production in target cells resulted in increased cytotoxicity in AEC and RPAEC. This study shows for the first time that target cells are more resistant to effector cells under hypoxia, suggesting hypoxia-induced cell protection. An underlying mechanism for this phenomenon might be the protective effect of increased levels of NO in target cells. |
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Authors:
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S Meyer; B R Z'graggen; S Blumenthal; A Borgeat; M T Ganter; L Reyes; C Booy; T A Neff; D R Spahn; B Beck-Schimmer |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-09-24 |
Journal Detail:
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Title: Clinical and experimental immunology Volume: 150 ISSN: 1365-2249 ISO Abbreviation: Clin. Exp. Immunol. Publication Date: 2007 Nov |
Date Detail:
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Created Date: 2007-10-16 Completed Date: 2007-12-18 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0057202 Medline TA: Clin Exp Immunol Country: England |
Other Details:
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Languages: eng Pagination: 358-67 Citation Subset: IM |
Affiliation:
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Institute of Anaesthesiology, and Institute of Physiology and Center for Intergrative Human Physiology, University of Zurich, Zurich, Switzerland. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion / physiology Cell Death / physiology Cell Hypoxia / physiology Cells, Cultured Endothelium, Vascular / cytology*, metabolism Epithelial Cells / metabolism, physiology Female HSP70 Heat-Shock Proteins / metabolism Humans Intercellular Adhesion Molecule-1 / biosynthesis, genetics, metabolism Neutrophils / metabolism, physiology* Nitric Oxide / biosynthesis Pulmonary Alveoli / cytology*, metabolism Pulmonary Artery / cytology*, metabolism RNA, Messenger / genetics Rats Reverse Transcriptase Polymerase Chain Reaction / methods Up-Regulation |
| Chemical | |
Reg. No./Substance:
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0/HSP70 Heat-Shock Proteins; 0/RNA, Messenger; 10102-43-9/Nitric Oxide; 126547-89-5/Intercellular Adhesion Molecule-1 |
| Comments/Corrections | |
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