Document Detail


Hypoxia-amplified proliferation of human dental pulp cells.
MedLine Citation:
PMID:  19482178     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
INTRODUCTION: Postnatal human dental pulp is a potentially promising source of progenitor cells. Sustaining and amplifying progenitor cell populations would be beneficial for basic science research with application in pulpal regeneration. Hypoxia has been observed to promote the undifferentiated cell state in various stem cell populations. The purpose of this study was to examine human dental pulp cells (DPCs) proliferation in normoxia and hypoxia. METHODS: Dental pulp cells were obtained from third molars of adult patients and cultured in alpha modification of Eagle's medium culture medium with 10% fetal bovine serum. For cell proliferation, DPCs were divided into two groups: (1) DPCs incubated in normoxic conditions (20% oxygen tension) and (2) DPC incubated in hypoxic conditions (3% oxygen tension). Cell proliferation assays were performed every 2 to 3 days from day 3 to day 14 by trypsinization and quantification of cells with a hemacytometer. Fluorescence-activated cell sorting analysis was completed to investigate stem cell markers, CD133, and STRO-1. RESULTS: DPCs proliferated significantly more in hypoxia than in normoxia (ie, two-fold throughout the experiment, p < 0.0001). The primitive stem cell marker, CD133, decreased in hypoxia, whereas the osteoprogenitor marker, STRO-1, increased by 8.5-fold. CONCLUSIONS: This study suggested that hypoxia is an effective treatment to amplify numbers of progenitor cells from human dental pulp.
Authors:
Jaruma Beau Sakdee; Robert R White; Tom C Pagonis; Peter V Hauschka
Related Documents :
17972108 - High hydrogen peroxide concentration in the feed-zone affects bioreactor cell productiv...
17303578 - Cytochrome c oxidase activity and oxygen tolerance.
8997208 - Oxygen dependence of respiration in coupled and uncoupled endothelial cells.
113548 - Isolation and separation of toad bladder epithelial cells.
3117578 - Neuroendocrine background of the pathology of the islets of langerhans. a minireview wi...
23129058 - Protective effects of hydrogen sulfide on oxidative stress and fibrosis in hepatic stel...
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-04-19
Journal Detail:
Title:  Journal of endodontics     Volume:  35     ISSN:  1878-3554     ISO Abbreviation:  J Endod     Publication Date:  2009 Jun 
Date Detail:
Created Date:  2009-06-01     Completed Date:  2009-08-17     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7511484     Medline TA:  J Endod     Country:  United States    
Other Details:
Languages:  eng     Pagination:  818-23     Citation Subset:  D    
Affiliation:
Department of Restorative Dentistry, Harvard School of Medicine, Boston, MA 02115, USA. bjsakdee@post.harvard.edu
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Adult
Antigens, CD / analysis
Antigens, Surface / analysis
Cell Count
Cell Hypoxia / physiology*
Cell Proliferation
Cell Separation
Cells, Cultured
Dental Pulp / cytology*
Flow Cytometry
Glycoproteins / analysis
Humans
Peptides / analysis
Stem Cells / cytology*
Chemical
Reg. No./Substance:
0/AC133 antigen; 0/Antigens, CD; 0/Antigens, Surface; 0/Glycoproteins; 0/Peptides; 0/STRO-1 antigen, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Furcation groove of maxillary first premolar, thickness, and dentin structures.
Next Document:  Endodontic chelators induce nitric oxide expression by murine-cultured macrophages.