| Hypoxia-amplified proliferation of human dental pulp cells. | |
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MedLine Citation:
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PMID: 19482178 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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INTRODUCTION: Postnatal human dental pulp is a potentially promising source of progenitor cells. Sustaining and amplifying progenitor cell populations would be beneficial for basic science research with application in pulpal regeneration. Hypoxia has been observed to promote the undifferentiated cell state in various stem cell populations. The purpose of this study was to examine human dental pulp cells (DPCs) proliferation in normoxia and hypoxia. METHODS: Dental pulp cells were obtained from third molars of adult patients and cultured in alpha modification of Eagle's medium culture medium with 10% fetal bovine serum. For cell proliferation, DPCs were divided into two groups: (1) DPCs incubated in normoxic conditions (20% oxygen tension) and (2) DPC incubated in hypoxic conditions (3% oxygen tension). Cell proliferation assays were performed every 2 to 3 days from day 3 to day 14 by trypsinization and quantification of cells with a hemacytometer. Fluorescence-activated cell sorting analysis was completed to investigate stem cell markers, CD133, and STRO-1. RESULTS: DPCs proliferated significantly more in hypoxia than in normoxia (ie, two-fold throughout the experiment, p < 0.0001). The primitive stem cell marker, CD133, decreased in hypoxia, whereas the osteoprogenitor marker, STRO-1, increased by 8.5-fold. CONCLUSIONS: This study suggested that hypoxia is an effective treatment to amplify numbers of progenitor cells from human dental pulp. |
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Authors:
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Jaruma Beau Sakdee; Robert R White; Tom C Pagonis; Peter V Hauschka |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-04-19 |
Journal Detail:
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Title: Journal of endodontics Volume: 35 ISSN: 1878-3554 ISO Abbreviation: J Endod Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-06-01 Completed Date: 2009-08-17 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 7511484 Medline TA: J Endod Country: United States |
Other Details:
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Languages: eng Pagination: 818-23 Citation Subset: D |
Affiliation:
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Department of Restorative Dentistry, Harvard School of Medicine, Boston, MA 02115, USA. bjsakdee@post.harvard.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult Antigens, CD / analysis Antigens, Surface / analysis Cell Count Cell Hypoxia / physiology* Cell Proliferation Cell Separation Cells, Cultured Dental Pulp / cytology* Flow Cytometry Glycoproteins / analysis Humans Peptides / analysis Stem Cells / cytology* |
| Chemical | |
Reg. No./Substance:
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0/AC133 antigen; 0/Antigens, CD; 0/Antigens, Surface; 0/Glycoproteins; 0/Peptides; 0/STRO-1 antigen, human |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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