| Hypoxia and amino acid supplementation synergistically promote the osteogenesis of human mesenchymal stem cells on silk protein scaffolds. | |
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MedLine Citation:
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PMID: 20673134 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Tailoring tissue engineering strategies to match patient- and tissue-specific bone regeneration needs offers to improve clinical outcomes. As a step toward this goal, osteogenic outcomes and metabolic parameters were assessed when varying inputs into the bone formation process. Silk protein scaffolds seeded with human mesenchymal stem cells in osteogenic differentiation media were used to study in vitro osteogenesis under varied conditions of amino acid (lysine and proline) concentration and oxygen level. The cells were assessed to probe how the microenvironment impacted metabolic pathways and thus osteogenesis. The most favorable osteogenesis outcomes were found in the presence of low (5%) oxygen combined with high lysine and proline concentrations during in vitro cultivation. This same set of culture conditions also showed the highest glucose consumption, lactate synthesis, and certain amino acid consumption rates. On the basis of these results and known pathways, a holistic metabolic model was derived which shows that lysine and proline supplements as well as low (5%) oxygen levels regulate collagen matrix synthesis and thereby rates of osteogenesis. This study establishes early steps toward a foundation for patient- and tissue-specific matches between metabolism, repair site, and tissue engineering approaches toward optimized bone regeneration. |
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Authors:
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Sejuti Sengupta; Sang-Hyug Park; Atur Patel; Julia Carn; Kyongbum Lee; David L Kaplan |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2010-09-01 |
Journal Detail:
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Title: Tissue engineering. Part A Volume: 16 ISSN: 1937-335X ISO Abbreviation: Tissue Eng Part A Publication Date: 2010 Dec |
Date Detail:
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Created Date: 2010-12-14 Completed Date: 2011-04-07 Revised Date: 2011-12-21 |
Medline Journal Info:
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Nlm Unique ID: 101466659 Medline TA: Tissue Eng Part A Country: United States |
Other Details:
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Languages: eng Pagination: 3623-34 Citation Subset: IM |
Affiliation:
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Department of Biomedical Engineering, School of Engineering, Tufts University, Medford, Massachusetts 02155, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acids
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chemistry,
pharmacology* Animals Cell Hypoxia / physiology* Cells, Cultured Chromatography, High Pressure Liquid Humans Immunohistochemistry Mesenchymal Stem Cells / cytology*, metabolism, physiology* Microscopy, Electron, Scanning Osteogenesis / drug effects* Silk / chemistry* Tissue Engineering / methods* Tissue Scaffolds / chemistry* |
| Grant Support | |
ID/Acronym/Agency:
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P41 EB002520/EB/NIBIB NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Amino Acids; 0/Silk |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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