| Hypoxia Differentially Reorganizes Contractile Proteins in Fetal and Adult Ovine Carotid Arteries. | |
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MedLine Citation:
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PMID: 23325408 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Chronic hypoxia induces vascular remodeling through mechanisms that remain poorly understood. Because hypoxia potently increases production of Vascular Endothelial Growth Factor (VEGF), which we have recently shown can modulate smooth muscle phenotype, the present study explores the hypothesis that VEGF contributes to hypoxic vascular remodeling through changes in the abundance, organization and function of contractile proteins. Adult and fetal sheep were acclimatized at sea level or at altitude (3280m) for the final 110 days of gestation. Carotid arteries from these animals were denuded of endothelium and used fresh or after organ culture in 3 ng/ml VEGF, to determine in vitro myogenic contractility, artery stiffness, and the abundances (determined via immunoblots) of Smooth Muscle α-Actin (SMαA), Myosin Light Chain Kinase (MLCK) and 20 kDa Regulatory Myosin Light Chain (MLC20). Artery sections were also fixed in 4% paraformaldehyde and sectioned for confocal colocalization analysis of contractile protein interactions. Hypoxia increased medial thickness and stiffness but decreased myogenic tone in fetal and adult arteries. Hypoxia increased SMαA and MLC20 abundances but decreased MLCK markedly in both fetal and adult arteries. The effects of VEGF and hypoxia on MLC20 abundance, and contractile protein colocalization in general, were similar in fetal arteries. Overall, the data strongly suggest that contractile protein abundance and organization are independently regulated, and that VEGF contributes to hypoxic vascular remodeling, but more so in fetal than adult arteries. |
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Authors:
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Olayemi O Adeoye; Stacy M Butler; Margaret C Hubbell; Andrew J Semotiuk; James M Williams; William J Pearce |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2013-1-16 |
Journal Detail:
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Title: American journal of physiology. Cell physiology Volume: - ISSN: 1522-1563 ISO Abbreviation: Am. J. Physiol., Cell Physiol. Publication Date: 2013 Jan |
Date Detail:
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Created Date: 2013-1-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100901225 Medline TA: Am J Physiol Cell Physiol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Loma Linda University School of Medicine. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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