Document Detail


Contribution of increased VEGF receptors to hypoxic changes in fetal ovine carotid artery contractile proteins.
MedLine Citation:
PMID:  23325408     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recent studies suggest that vascular endothelial growth factor (VEGF) can modulate smooth muscle phenotype and, consequently, the composition and function of arteries upstream from the microcirculation, where angiogenesis occurs. Given that hypoxia potently induces VEGF, the present study explores the hypothesis that, in fetal arteries, VEGF contributes to hypoxic vascular remodeling through changes in abundance, organization, and function of contractile proteins. Pregnant ewes were acclimatized at sea level or at altitude (3,820 m) for the final 110 days of gestation. Endothelium-denuded carotid arteries from full-term fetuses were used fresh or after 24 h of organ culture in a physiological concentration (3 ng/ml) of VEGF. After 110 days, hypoxia had no effect on VEGF abundance but markedly increased abundance of the Flk-1 (171%) and Flt-1 (786%) VEGF receptors. Hypoxia had no effect on smooth muscle α-actin (SMαA), decreased myosin light chain (MLC) kinase (MLCK), and increased 20-kDa regulatory MLC (MLC(20)) abundances. Hypoxia also increased MLCK-SMαA, MLC(20)-SMαA, and MLCK-MLC(20) colocalization. Compared with hypoxia, organ culture with VEGF produced the same pattern of changes in contractile protein abundance and colocalization. Effects of VEGF on colocalization were blocked by the VEGF receptor antagonists vatalanib (240 nM) and dasatinib (6.3 nM). Thus, through increases in VEGF receptor density, hypoxia can recruit VEGF to help mediate remodeling of fetal arteries upstream from the microcirculation. The results support the hypothesis that VEGF contributes to hypoxic vascular remodeling through changes in abundance, organization, and function of contractile proteins.
Authors:
Olayemi O Adeoye; Stacy M Butler; Margaret C Hubbell; Andrew Semotiuk; James M Williams; William J Pearce
Related Documents :
22959288 - Extrapleural harvesting of the mammary artery and the costodiaphragmatic sulcus.
12537228 - Late quadriplegia after adult coarctation repair.
23443338 - Anomalous great cardiac vein draining into the right atrium combined with a single left...
9119518 - Angiographic findings of takayasu arteritis: new classification.
18487538 - Cerebral arteries: fully automated segmentation from ct angiography--a feasibility study.
15236038 - Effects of alveolar hypoxia on pulmonary capillary beds.
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't     Date:  2013-01-16
Journal Detail:
Title:  American journal of physiology. Cell physiology     Volume:  304     ISSN:  1522-1563     ISO Abbreviation:  Am. J. Physiol., Cell Physiol.     Publication Date:  2013 Apr 
Date Detail:
Created Date:  2013-04-02     Completed Date:  2013-05-24     Revised Date:  2014-04-01    
Medline Journal Info:
Nlm Unique ID:  100901225     Medline TA:  Am J Physiol Cell Physiol     Country:  United States    
Other Details:
Languages:  eng     Pagination:  C656-65     Citation Subset:  IM    
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Animals
Carotid Arteries / embryology,  metabolism*
Cell Hypoxia
Contractile Proteins / genetics,  metabolism*
Female
Fetus / metabolism
Gene Expression Regulation, Developmental / physiology
Neovascularization, Physiologic
Pregnancy
Protein Transport
Receptors, Vascular Endothelial Growth Factor / genetics,  metabolism*
Sheep
Tissue Culture Techniques
Vascular Endothelial Growth Factor A / genetics,  metabolism
Vasoconstriction
Grant Support
ID/Acronym/Agency:
HD-31266/HD/NICHD NIH HHS; HL-54120/HL/NHLBI NIH HHS; HL-64867/HL/NHLBI NIH HHS; NS-076945/NS/NINDS NIH HHS; P01 HD031226/HD/NICHD NIH HHS; R01 HL054120/HL/NHLBI NIH HHS; R01 NS076945/NS/NINDS NIH HHS
Chemical
Reg. No./Substance:
0/Contractile Proteins; 0/Vascular Endothelial Growth Factor A; EC 2.7.10.1/Receptors, Vascular Endothelial Growth Factor
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  Airway epithelium-derived relaxing factor: myth, reality or naivety?
Next Document:  Physiology of SLC12 transporters: lessons from inherited human genetic mutations and genetically eng...