| Hypoxia and CoCl2 protect HepG2 cells against serum deprivation- and t-BHP-induced apoptosis: a possible anti-apoptotic role for HIF-1. | |
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MedLine Citation:
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PMID: 15093734 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hypoxia inducible factor-1 (HIF-1) is the main transcriptional factor activated by hypoxia. Besides the well-described role assigned to HIF-1 in the adaptation of cells to hypoxia, different recent data describe a possible role for HIF-1 in the modulation of apoptosis. However, this precise role is not yet clearly understood. In this study, chemical and physiological hypoxia, which were shown to induce HIF-1alpha stabilization and HIF-1 activation, were shown to inhibit apoptosis induced in HepG2 cells by two different pro-apoptotic conditions, serum deprivation- and t-BHP-induced oxidative stress. Indeed, hypoxia reduced DNA fragmentation, caspase activation, and PARP cleavage induced by these two pro-apoptotic conditions. These results are very interesting because it is a clear demonstration that hypoxia and chemical hypoxia have a direct protective effect on apoptotic cell death induced by two different stimuli. This observation is an important data in understanding how tumor growth can occur in challenging environmental conditions. |
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Authors:
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Jean-Pascal Piret; Christophe Lecocq; Sebastien Toffoli; Noelle Ninane; Martine Raes; Carine Michiels |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Experimental cell research Volume: 295 ISSN: 0014-4827 ISO Abbreviation: Exp. Cell Res. Publication Date: 2004 May |
Date Detail:
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Created Date: 2004-04-19 Completed Date: 2004-05-20 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0373226 Medline TA: Exp Cell Res Country: United States |
Other Details:
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Languages: eng Pagination: 340-9 Citation Subset: IM |
Affiliation:
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Laboratory of Biochemistry and Cellular Biology, Facultés Universitaires Notre-Dame de la Paix, 5000 Namur, Belgium. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Apoptosis
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drug effects,
physiology* Blotting, Western Carcinoma, Hepatocellular Caspases / metabolism Cell Hypoxia / physiology* Cell Line, Tumor Cobalt / toxicity* Culture Media, Serum-Free DNA-Binding Proteins / metabolism* Enzyme Activation Genes, Reporter Humans Hypoxia-Inducible Factor 1 Hypoxia-Inducible Factor 1, alpha Subunit Liver Neoplasms Nuclear Proteins / metabolism* Oxidative Stress / drug effects, physiology Polymerase Chain Reaction Transcription Factors / physiology tert-Butylhydroperoxide / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Culture Media, Serum-Free; 0/DNA-Binding Proteins; 0/HIF1A protein, human; 0/Hypoxia-Inducible Factor 1; 0/Hypoxia-Inducible Factor 1, alpha Subunit; 0/Nuclear Proteins; 0/Transcription Factors; 7440-48-4/Cobalt; 75-91-2/tert-Butylhydroperoxide; 7646-79-9/cobaltous chloride; EC 3.4.22.-/Caspases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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