| Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants. | |
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MedLine Citation:
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PMID: 6294614 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Recombinant plasmids containing DNA inserts complementary to mRNA coding for hypoxanthine-guanine phosphoribosyltransferase (HPRT) from mouse and Chinese hamster cell lines have been isolated from cDNA libraries and characterized by DNA sequence analysis. A total of 1292 nucleotides of the mouse cDNA sequence and 1301 nucleotides of the Chinese hamster cDNA sequence has been determined. Each of these sequences includes an open reading frame of 654 nucleotides (218 amino acids) corresponding to the HPRT protein coding region. The deduced amino acid sequences for the mouse and Chinese hamster enzymes are presented and compared to that of human HPRT. At least 95% of the amino acids are conserved in the three species. In addition, we present evidence that two species of HPRT mRNA, which differ in the site of polyadenylation that is utilized during processing of the RNA transcripts, exist in Chinese hamster cells. |
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Authors:
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D S Konecki; J Brennand; J C Fuscoe; C T Caskey; A C Chinault |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Nucleic acids research Volume: 10 ISSN: 0305-1048 ISO Abbreviation: Nucleic Acids Res. Publication Date: 1982 Nov |
Date Detail:
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Created Date: 1983-02-25 Completed Date: 1983-02-25 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0411011 Medline TA: Nucleic Acids Res Country: ENGLAND |
Other Details:
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Languages: eng Pagination: 6763-75 Citation Subset: IM |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/J00060; J00423 |
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cell Line Cloning, Molecular Cricetinae Cricetulus DNA / metabolism* DNA Restriction Enzymes DNA, Recombinant / metabolism* Genes* Hypoxanthine Phosphoribosyltransferase / genetics* Lung Mice Neuroblastoma Plasmids* RNA, Messenger / genetics |
| Grant Support | |
ID/Acronym/Agency:
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GMO7526/GM/NIGMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/DNA, Recombinant; 0/RNA, Messenger; 9007-49-2/DNA; EC 2.4.2.8/Hypoxanthine Phosphoribosyltransferase; EC 3.1.21.-/DNA Restriction Enzymes |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
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Journal Information Journal ID (nlm-ta): Nucleic Acids Res ISSN: 0305-1048 ISSN: 1362-4962 |
Article Information Download PDF  Print publication date: Day: 11 Month: 11 Year: 1982 Volume: 10 Issue: 21 First Page: 6763 Last Page: 6775 ID: 326962 PubMed Id: 6294614 |
| Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants. | |
| D S Konecki | |
| J Brennand | |
| J C Fuscoe | |
| C T Caskey | |
| A C Chinault | |
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