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Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants.
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MedLine Citation:
PMID:  6294614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Recombinant plasmids containing DNA inserts complementary to mRNA coding for hypoxanthine-guanine phosphoribosyltransferase (HPRT) from mouse and Chinese hamster cell lines have been isolated from cDNA libraries and characterized by DNA sequence analysis. A total of 1292 nucleotides of the mouse cDNA sequence and 1301 nucleotides of the Chinese hamster cDNA sequence has been determined. Each of these sequences includes an open reading frame of 654 nucleotides (218 amino acids) corresponding to the HPRT protein coding region. The deduced amino acid sequences for the mouse and Chinese hamster enzymes are presented and compared to that of human HPRT. At least 95% of the amino acids are conserved in the three species. In addition, we present evidence that two species of HPRT mRNA, which differ in the site of polyadenylation that is utilized during processing of the RNA transcripts, exist in Chinese hamster cells.
Authors:
D S Konecki; J Brennand; J C Fuscoe; C T Caskey; A C Chinault
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Nucleic acids research     Volume:  10     ISSN:  0305-1048     ISO Abbreviation:  Nucleic Acids Res.     Publication Date:  1982 Nov 
Date Detail:
Created Date:  1983-02-25     Completed Date:  1983-02-25     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0411011     Medline TA:  Nucleic Acids Res     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  6763-75     Citation Subset:  IM    
Data Bank Information
Bank Name/Acc. No.:
GENBANK/J00060;  J00423
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MeSH Terms
Descriptor/Qualifier:
Animals
Base Sequence
Cell Line
Cloning, Molecular
Cricetinae
Cricetulus
DNA / metabolism*
DNA Restriction Enzymes
DNA, Recombinant / metabolism*
Genes*
Hypoxanthine Phosphoribosyltransferase / genetics*
Lung
Mice
Neuroblastoma
Plasmids*
RNA, Messenger / genetics
Grant Support
ID/Acronym/Agency:
GMO7526/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/DNA, Recombinant; 0/RNA, Messenger; 9007-49-2/DNA; EC 2.4.2.8/Hypoxanthine Phosphoribosyltransferase; EC 3.1.21.-/DNA Restriction Enzymes
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Full Text
Journal Information
Journal ID (nlm-ta): Nucleic Acids Res
ISSN: 0305-1048
ISSN: 1362-4962
Article Information
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Print publication date: Day: 11 Month: 11 Year: 1982
Volume: 10 Issue: 21
First Page: 6763 Last Page: 6775
ID: 326962
PubMed Id: 6294614

Hypoxanthine-guanine phosphoribosyltransferase genes of mouse and Chinese hamster: construction and sequence analysis of cDNA recombinants.
D S Konecki
J Brennand
J C Fuscoe
C T Caskey
A C Chinault



Article Categories:
  • Research Article


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