Document Detail


Hypothermic preservation effect on mammalian cells of type III antifreeze proteins from notched-fin eelpout.
MedLine Citation:
PMID:  18603237     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Antifreeze proteins (AFPs) can bind to the surface of ice crystals and have also been suggested to protect cells from hypothermic damage. The present study reports that type III AFPs from notched-fin eelpout, Zoarces elongatus Kner, can protect cells during hypothermic storage. This fish naturally expresses at least 13 isoforms of type III AFP (denoted NfeAFPs), the primary sequences of which were categorized into SP- and QAE-Sephadex binding groups (SP- and QAE-isoforms). We compared the preservation ability between the extracted isoform mixtures (NfeAFPs) and a recombinant single SP-isoform (RcNfeAFP6). Experiments were performed using cultivated mammalian cells (HepG2) exposed to 4 degrees C for 24-72 h. The preserved cells were evaluated by measuring LDH released, intracellular ATP, and WST-8 reduction. It appeared that the protective effect of the 2 samples increases dose-dependently at concentrations between 2 and 10 mg/ml. Under highest soluble amount of the protein (approximately 10 mg/ml), cell viability significantly improved compared with the ordinary preservation fluid (P<0.01). This effect was larger with NfeAFPs than with RcNfeAFP6 at the same concentration. The successful hypothermic preservation of cells using natural NfeAFPs may have a wide range of applications for cell engineering and clinical medical care.
Authors:
Yu Hirano; Yoshiyuki Nishimiya; Shuichiro Matsumoto; Michiaki Matsushita; Satoru Todo; Ai Miura; Yasuo Komatsu; Sakae Tsuda
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, Non-U.S. Gov't     Date:  2008-06-04
Journal Detail:
Title:  Cryobiology     Volume:  57     ISSN:  1090-2392     ISO Abbreviation:  Cryobiology     Publication Date:  2008 Aug 
Date Detail:
Created Date:  2008-08-01     Completed Date:  2008-10-31     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0006252     Medline TA:  Cryobiology     Country:  United States    
Other Details:
Languages:  eng     Pagination:  46-51     Citation Subset:  IM    
Affiliation:
Research Institute of Genome Based Biofactory, National Institute of Advanced Industrial Science and Technology, 2-17-2-1 Tsukisamu-Higashi, Toyohira, Sapporo, Hokkaido 062-8517, Japan.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antifreeze Proteins, Type III / metabolism*,  pharmacology
Cells, Cultured
Cryopreservation*
Fish Proteins / metabolism*,  pharmacology
Humans
Perciformes / classification,  metabolism*
Protein Isoforms / metabolism,  pharmacology
Recombinant Proteins / metabolism,  pharmacology
Chemical
Reg. No./Substance:
0/Antifreeze Proteins, Type III; 0/Fish Proteins; 0/Protein Isoforms; 0/Recombinant Proteins

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