Document Detail

Hypothermia reduces secretion of vascular endothelial growth factor by cultured retinal pigment epithelial cells.
MedLine Citation:
PMID:  20805126     Owner:  NLM     Status:  In-Process    
AIM: Visual loss in age-related macular degeneration usually develops secondary to choroidal neovascularisation. Vascular endothelial growth factor (VEGF) is a critical regulator of retinal angiogenesis and vascular permeability, especially in hypoxic conditions. We hypothesise that hypothermia may reduce the retinal pigment epithelium (RPE) metabolism and, consequently, the levels of VEGF secretion by cultured RPE cells under hypoxic conditions. The effects of hypothermia were compared with the metabolic inhibiting effects of thiopental and nicotinamide.
METHODS: ARPE-19 cells were grown in culture for up to 5 days under normoxic (20% O(2)) and hypoxic (1% O(2)) conditions at temperatures ranging from 27°C to 40°C. For experiments with pharmacological agents, thiopental and nicotinamide were added to the media. VEGF levels in the media were measured by ELISA and cell metabolic activity was measured by a fluorescent cell metabolic assay.
RESULTS: We found that hypothermia reduced ARPE-19 cell metabolism in a temperature-dependent fashion. Hypothermia also reduced ARPE-19 cell VEGF secretion in a temperature-dependent fashion. ARPE-19 cell VEGF secretion was reduced by 38% at 34°C compared with cells grown at 37°C. Conversely, ARPE-19 cell VEGF secretion was increased by 32% at 40°C compared with cells grown at 37°C. Hypoxia increased ARPE-19 cell VEGF secretion by 84% at 37°C. However, hypothermia decreased the hypoxia-induced increase of ARPE-19 cell VEGF secretion by 30%. The effect of hypothermia on ARPE-19 cell VEGF secretion was reversible after 4 days. In contrast to hypothermia, thiopental and nicotinamide were able to reduce RPE cell metabolism but not VEGF secretion.
CONCLUSION: Hypothermia decreases both VEGF secretion and cellular metabolism in ARPE-19 cells. Hypothermia also mitigates the hypoxia-induced increase in ARPE-19 cell VEGF secretion. These effects of hypothermia are potentially unique and cannot be obtained by other pharmacological agents that slow cellular metabolism.
Marco Coassin; Keith G Duncan; Kathy R Bailey; Ajay Singh; Daniel M Schwartz
Related Documents :
16963616 - Response of mitochondrial reactive oxygen species generation to steady-state oxygen ten...
19395246 - Enhanced growth inhibition effect of resveratrol incorporated into biodegradable nanopa...
9387096 - Aerobic glycolysis by proliferating cells: protection against oxidative stress at the e...
18634086 - Inducing single-cell suspension of bti-tn5b1-4 insect cells: i. the use of sulfated pol...
8264336 - Control of neoplastic cell proliferation and differentiation by restoration of 4-hydrox...
21968386 - Synergistic interactions of saponins and monoterpenes in hela cells, cos7 cells and in ...
Publication Detail:
Type:  Journal Article     Date:  2010-08-30
Journal Detail:
Title:  The British journal of ophthalmology     Volume:  94     ISSN:  1468-2079     ISO Abbreviation:  Br J Ophthalmol     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-11-24     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0421041     Medline TA:  Br J Ophthalmol     Country:  England    
Other Details:
Languages:  eng     Pagination:  1678-83     Citation Subset:  IM    
Department of Ophthalmology, University of California, San Francisco, CA 94143-0730, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Comparing the ganglion cell complex and retinal nerve fibre layer measurements by Fourier domain OCT...
Next Document:  Factors that correlate with improvement in vision after combined Intacs and trans-epithelial corneal...