| Hypothermia and anesthetic postconditioning influence the expression and activity of small intestinal proteins possibly involved in ischemia/reperfusion-mediated events following cardiopulmonary resuscitation. | |
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MedLine Citation:
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PMID: 21763251 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Aim of the study: Successful resuscitation after cardiac arrest is typically associated with cerebral and myocardial ischemia/reperfusion (I/R)-injury. Recently, we have demonstrated effects of therapeutic hypothermia (HT) and postconditioning with the volatile anesthetic sevoflurane (SEV) on I/R-mediated mechanisms in the heart and brain (Meybohm et al, PLoS One, 2009; Meybohm et al, Crit Care, 2010). As the intestine is also highly susceptible to I/R-injury, we investigated the influence of HT and SEV on intestinal I/R-mediated events induced by cardiac arrest and successful resuscitation. Methods: Effects of I/R, HT (12h, 33°C) and a combination of HT with SEV (12h, 2.0 vol%) were evaluated in a pig model of cardiac arrest and successful cardiopulmonary resuscitation. Westernblotting, ELISA, caspase-3/7 assays, myeloperoxidase (MPO) quantifications and gelatine zymography were performed using intestinal tissue derived 24hours after return of spontaneous circulation. Results: Compared to the normothermia control, HT and HT+SEV resulted in a significant increase in intestinal HIF-1α protein expression (P<0.05). Tissue concentrations of IL-1β were significantly reduced in the HT and HT+SEV group (P<0.05), whereas a reduction of IL-10 levels was only detected in the intestine of animals treated with HT+SEV (P<0.05). A statistically significant increase of intestinal MPO activity was found in the HT+SEV group (P<0.01). Activities of caspase-3 and 7 or matrixmetalloproteinase-2 were not changed in any of the groups investigated, the activity of matrixmetalloproteinase-9 was, however, significantly increased in the HT+SEV group (P<0.05). Conclusion: HT and postconditioning with SEV influence the expression and activity of several small intestinal proteins that are possibly involved in intestinal I/R-mediated events following successful cardiopulmonary resuscitation. |
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Authors:
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Martin Albrecht; Matthias Gruenewald; Karina Zitta; Kai Zacharowski; Jens Scholz; Berthold Bein; Patrick Meybohm |
Publication Detail:
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Type: JOURNAL ARTICLE Date: 2011-7-13 |
Journal Detail:
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Title: Resuscitation Volume: - ISSN: 1873-1570 ISO Abbreviation: - Publication Date: 2011 Jul |
Date Detail:
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Created Date: 2011-7-18 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0332173 Medline TA: Resuscitation Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Copyright Information:
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Copyright © 2011. Published by Elsevier Ireland Ltd. |
Affiliation:
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Department of Anaesthesiology and Intensive Care Medicine, University Hospital Schleswig-Holstein, Campus Kiel, Germany. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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