Document Detail

Hypothalamic neuropeptide expression after food restriction in Zucker rats: evidence of persistent neuropeptide Y gene activation.
MedLine Citation:
PMID:  1363327     Owner:  NLM     Status:  MEDLINE    
The Obese Zucker rat is a model of genetic obesity characterized by hyperphagia, hyperinsulinemia and other endocrine abnormalities. In order to elucidate pathogenetic mechanisms contributing to disturbed feeding behavior in these animals, the effect of food restriction on three hypothalamic neuropeptides involved in the control of food intake was studied. Eighteen male obese and 18 lean Zucker rats were randomly divided into two groups: half of the animals were food-restricted for 2 weeks, while the other half served as controls and were fed ad libitum. The levels of preproneuropeptide Y (preproNPY), preprocorticotropin releasing factor (preproCRF) and preprosomatostatin (preproSOM) mRNAs were determined using in situ hybridization technique. In addition, plasma insulin and corticosterone concentrations were analyzed. Food restriction significantly increased the expression of preproNPY mRNA in the arcuate nucleus in both Zucker phenotypes, while the expressions of preproCRF mRNA in the paraventricular nucleus (PVN) and preproSOM mRNA in the periventricular nucleus (PeV) were not altered. The expression of preproNPY mRNA was significantly greater in control obese animals compared to control lean animals. Food restriction lowered plasma insulin levels, but did not change plasma corticosterone levels. It is concluded that food restriction specifically activates NPY gene transcription in the arcuate nucleus the response being similar in both Zucker phenotypes. The results suggest that orexigenic NPY plays a role in the adaptation to altered feeding status.
U Pesonen; R Huupponen; J Rouru; M Koulu
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Brain research. Molecular brain research     Volume:  16     ISSN:  0169-328X     ISO Abbreviation:  Brain Res. Mol. Brain Res.     Publication Date:  1992 Dec 
Date Detail:
Created Date:  1993-04-01     Completed Date:  1993-04-01     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  8908640     Medline TA:  Brain Res Mol Brain Res     Country:  NETHERLANDS    
Other Details:
Languages:  eng     Pagination:  255-60     Citation Subset:  IM    
Department of Pharmacology, University of Turku, Finland.
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MeSH Terms
Blood Glucose / metabolism
Corticotropin-Releasing Hormone / genetics
Food Deprivation / physiology*
Gene Expression Regulation / physiology*
Hypothalamus / metabolism*
Image Processing, Computer-Assisted
In Situ Hybridization
Insulin / blood
Neuropeptide Y / biosynthesis,  genetics*
Obesity / genetics*,  metabolism
Protein Precursors / genetics
Rats, Zucker
Somatostatin / genetics
Time Factors
Transcriptional Activation
Reg. No./Substance:
0/Blood Glucose; 0/Neuropeptide Y; 0/Protein Precursors; 0/pro-corticotropin releasing hormone; 11061-68-0/Insulin; 51110-01-1/Somatostatin; 9015-71-8/Corticotropin-Releasing Hormone; 92307-59-0/preproneuropeptide Y

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