Document Detail

Hypothalamic administration of cAMP agonist/PKA activator inhibits both schedule feeding and NPY-induced feeding in rats.
MedLine Citation:
PMID:  12668209     Owner:  NLM     Status:  MEDLINE    
Following central administration, neuropeptides that decrease the level of cAMP induce feeding. Conversely, cAMP activating neuropeptides tend to elicit satiety. When the inhibitory effect of neuropeptide Y (NPY) on the hypothalamic cAMP production was blocked by pertussis toxin, the potent orexigenic effect of NPY was lost. These findings suggest that there may be a link between hypothalamic cAMP and the central regulation of food intake. In this report, we show that the injection of the membrane-permeable cAMP agonist, adenosine-3',5'-cyclic monophosphorothioate Sp-isomer (Sp-cAMP), into perifornical hypothalamus (PFH) significantly inhibited schedule-induced and NPY-induced food intake for up to 4h. This inhibitory effect was normalized within 24h. A taste aversion could not be conditioned to Sp-cAMP treatment, suggesting that the anorectic response was not due to malaise. Sp-cAMP administration significantly increased the active protein kinase A (PKA) activity in dorsomedial (DMH) and ventromedial (VMH), but not in lateral (LH) hypothalamus. Consistently, food deprivation lowered, while refeeding normalized endogenous cAMP content in DMH and VMH, but not in LH areas. No significant effect of adenosine-3',5'-cyclic monophosphorothioate Rp-isomer (Rp-cAMP, cAMP antagonist) was observed on hypothalamic PKA activity, schedule-induced, or NPY-induced food intake. These findings suggest that the increase in cAMP level and PKA activity in DMH and VMH areas may trigger a satiety signal.
Sulaiman Sheriff; William T Chance; Sabahat Iqbal; Tilat A Rizvi; Chun Xiao; John W Kasckow; A Balasubramaniam
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, Non-P.H.S.; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Peptides     Volume:  24     ISSN:  0196-9781     ISO Abbreviation:  Peptides     Publication Date:  2003 Feb 
Date Detail:
Created Date:  2003-04-01     Completed Date:  2004-01-12     Revised Date:  2007-11-15    
Medline Journal Info:
Nlm Unique ID:  8008690     Medline TA:  Peptides     Country:  United States    
Other Details:
Languages:  eng     Pagination:  245-54     Citation Subset:  IM    
Department of Surgery, College of Medicine, University of Cincinnati, 231 Albert Sabin Way, 45267, Cincinnati, OH, USA.
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MeSH Terms
Cyclic AMP / analogs & derivatives*,  metabolism,  pharmacology*
Cyclic AMP-Dependent Protein Kinases / metabolism*
Eating / drug effects*
Enzyme Activation / drug effects
Feeding Behavior / drug effects*
Hypothalamus / drug effects*,  metabolism
Neuropeptide Y / pharmacology*
Radioimmunoassay / methods
Rats, Sprague-Dawley
Thionucleotides / pharmacology
Time Factors
Grant Support
Reg. No./Substance:
0/Neuropeptide Y; 0/Thionucleotides; 23645-17-2/adenosine-3',5'-cyclic phosphorothioate; 60-92-4/Cyclic AMP; EC AMP-Dependent Protein Kinases

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