Document Detail

Hypothalamic hamartomas: neuropathological features with and without prior gamma knife radiosurgery.
MedLine Citation:
PMID:  23207720     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: The neuropathological consequences of Gamma Knife radiosurgery (GK) on hypothalamic hamartoma (HH) are unknown.
OBJECTIVE: In a cohort of patients undergoing surgery for treatment-resistant epilepsy, we compared surgically resected HH tissue from patients without (group I; n = 19) and with (group II; n = 10) a history of GK (median dose 16 Gy to the 50% isodose margin).
METHODS: Techniques included thick-section stereology for total nucleated and total neuron cell counts, and thin-section immunohistochemistry. Normal human hypothalamus derived from age-matched autopsy material was used as control tissue for CD68 immunohistochemistry. Qualitative scoring of tissue sections was performed by a neuropathologist who was blind to the GK treatment history.
RESULTS: GK is associated with decreased total cell density (p < 0.02). A dose-dependent association of GK with decreased total neuron density approached significance (p = 0.06). Group II HH tissue had significantly more (1) reactive gliosis, (2) thickened capillary endothelium and (3) microglial activation. Degenerative features, including karyorrhexis and pyknotic nuclei, were infrequent in group II and absent in group I HH tissue.
CONCLUSIONS: Nonnecrotizing doses of GK radiosurgery decrease cell density in human HH tissue. Cell loss resulting from GK may contribute to decreased excitation in the neuronal networks responsible for seizure onset in HH tissue.
John F Kerrigan; Angela Parsons; Stephen G Rice; Kristina Simeone; Andrew G Shetter; Adib A Abla; Erin Prenger; Stephen W Coons
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2012-11-29
Journal Detail:
Title:  Stereotactic and functional neurosurgery     Volume:  91     ISSN:  1423-0372     ISO Abbreviation:  Stereotact Funct Neurosurg     Publication Date:  2013  
Date Detail:
Created Date:  2013-02-08     Completed Date:  2013-07-23     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  8902881     Medline TA:  Stereotact Funct Neurosurg     Country:  Switzerland    
Other Details:
Languages:  eng     Pagination:  45-55     Citation Subset:  IM    
Copyright Information:
Copyright © 2012 S. Karger AG, Basel.
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MeSH Terms
Anticonvulsants / therapeutic use
Cell Count
Cell Death
Cell Nucleus / ultrastructure
Child, Preschool
Combined Modality Therapy
Endothelium, Vascular / pathology
Epilepsy / drug therapy,  etiology,  surgery
Gliosis / etiology,  pathology
Hamartoma / complications,  pathology*,  surgery
Hypothalamic Diseases / complications,  pathology*,  surgery
Microglia / pathology
Neurons / pathology
Postoperative Complications / etiology,  pathology
Retrospective Studies
Single-Blind Method
Young Adult
Reg. No./Substance:

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