| Hypoglycemic effect of Ganoderma lucidum polysaccharides. | |
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MedLine Citation:
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PMID: 14769208 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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AIM: To investigate the hypoglycemic effect of Ganoderma lucidum polysaccharides (Gl-PS) in the normal fasted mice and its possible mechanism. METHODS: Normal fasted mice were given a single dose of Gl-PS 25, 50, and 100 mg/kg by i.p. and the serum glucose was measured at 0, 3, and 6 h after administration. Gl-PS 100 mg/kg were also given by i.p. and the serum glucose and insulin levels were measured at 0 min, 30 min, 1 h, 3 h, 6 h, and 12 h. Pancreatic islets were isolated and incubated with glucose 5.6 mmol/L and different concentration of Gl-PS, the insulin content of islets and insulin release were examined. The islets fluorescent intensity of [Ca2+]i was also studied with a confocal microscope. Verapamil and egtazic acid were used to testify whether the insulin-releasing effect of Gl-PS was mediated by its ability to raise the Ca2+ influx. RESULTS: Gl-PS dose-dependently lowered the serum glucose levels at 3 h and 6 h after administration. Gl-PS 100 mg/kg raised the circulating insulin levels at 1 h after administration. In vitro, Gl-PS had no effect on islets insulin content, but it stimulated the insulin release after incubation with glucose 5.6 mmol/L. Confocal microscope showed that Gl-PS 100 mg/L had the capacity to raise the [Ca2+]i. The insulin-releasing effect of Gl-PS was inhibited by verapamil/egtazic acid. CONCLUSION: Gl-PS possesses the hypoglycemic effect on normal mice; one mechanism is through its insulin-releasing activity due to a facilitation of Ca2+ inflow to the pancreatic beta cells. |
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Authors:
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Hui-na Zhang; Zhi-bin Lin |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Acta pharmacologica Sinica Volume: 25 ISSN: 1671-4083 ISO Abbreviation: Acta Pharmacol. Sin. Publication Date: 2004 Feb |
Date Detail:
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Created Date: 2004-02-10 Completed Date: 2004-07-30 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 100956087 Medline TA: Acta Pharmacol Sin Country: China |
Other Details:
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Languages: eng Pagination: 191-5 Citation Subset: IM |
Affiliation:
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Department of Pharmacology, School of Basic Medical Sciences, Peking University, Beijing 100083, China. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Glucose / metabolism* Calcium / metabolism* Hypoglycemic Agents / isolation & purification, pharmacology* Insulin / metabolism Islets of Langerhans / metabolism Male Mice Plants, Medicinal / chemistry Polysaccharides / isolation & purification, pharmacology* Rats Rats, Wistar Reishi* / chemistry |
| Chemical | |
Reg. No./Substance:
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0/Blood Glucose; 0/Hypoglycemic Agents; 0/Polysaccharides; 11061-68-0/Insulin; 7440-70-2/Calcium |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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