Document Detail


Hypoglycaemia induces decreased islet blood perfusion mediated by the central nervous system in normal and Type 2 diabetic GK rats.
MedLine Citation:
PMID:  12830382     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIMS/HYPOTHESIS: The aim of this study was to evaluate the effects of induced hypoglycaemia on pancreatic-islet blood flow in normal rats and in the GK rat, an animal model of Type 2 diabetes which normally has an increased islet blood perfusion. METHODS: A 50% reduction in blood glucose concentrations was achieved by intravenous administration of a rapidly acting insulin (15 IU/kg body weight). Blood flows were measured by a non-radioactive microsphere technique. RESULTS: A pronounced decrease in islet blood flow was observed in all animals, but preferentially in the Type 2 diabetic GK rats. When a similar dose of insulin was given to whole-pancreas transplanted rats only islet blood flow in the native pancreas was decreased, whereas that of the transplanted, i.e. denervated, pancreas was unchanged. Administration of 2-deoxy-D-glucose, which induces intracellular glucopenia especially in neurons, also decreased islet blood flow despite a systemic hyperglycaemia. CONCLUSION/INTERPRETATION: Hypoglycaemia leads to a preferential decrease in pancreatic-islet blood perfusion. The effect is probably mediated by the central nervous system, since 2-deoxy D-glucose-induced neuronal glucopenia caused a similar decrease in blood flow. The effects of islet blood flow are not likely to be mediated by nervous stimulation of the adrenal glands, with an associated release of catecholamines, because the transplanted pancreas was not affected by hypoglycaemia. The decreased islet blood perfusion could possibly diminish the output of insulin from the islets, thereby preventing a further decrease in blood glucose concentrations.
Authors:
P-O Carlsson; C Berne; C-G Ostenson; A Andersson; L Jansson
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2003-06-26
Journal Detail:
Title:  Diabetologia     Volume:  46     ISSN:  0012-186X     ISO Abbreviation:  Diabetologia     Publication Date:  2003 Aug 
Date Detail:
Created Date:  2003-08-08     Completed Date:  2004-05-14     Revised Date:  2010-03-24    
Medline Journal Info:
Nlm Unique ID:  0006777     Medline TA:  Diabetologia     Country:  Germany    
Other Details:
Languages:  eng     Pagination:  1124-30     Citation Subset:  IM    
Affiliation:
Department of Medical Cell Biology, Biomedical Centre, Box 571, 751 23, Uppsala, Sweden. Per-Ola.Carlsson@medcellbiol.uu.se
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MeSH Terms
Descriptor/Qualifier:
Animals
Blood Flow Velocity / drug effects
Blood Glucose / metabolism
Diabetes Mellitus, Type 2 / blood,  physiopathology*,  surgery
Duodenum / transplantation
Hypoglycemia / physiopathology*
Insulin / pharmacology
Islets of Langerhans / blood supply*
Male
Pancreas Transplantation / physiology
Perfusion
Rats
Rats, Inbred Strains
Rats, Inbred WF
Rats, Sprague-Dawley
Rats, Wistar
Reference Values
Transplantation, Homologous
Chemical
Reg. No./Substance:
0/Blood Glucose; 11061-68-0/Insulin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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