Document Detail


Hypocholesterolemic activity of grape seed proanthocyanidin is mediated by enhancement of bile acid excretion and up-regulation of CYP7A1.
MedLine Citation:
PMID:  20092993     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Interest in grape seed proanthocyanidin (GSP) as a cholesterol-lowering nutraceutical is growing. This study was to investigate the effect of GSP on blood cholesterol level and gene expression of cholesterol-regulating enzymes in Golden Syrian hamsters maintained on a 0.1% cholesterol diet. Results affirmed supplementation of 0.5% or 1.0% GSP could decrease plasma total cholesterol and triacylglycerol level. Western blot and real-time polymerase chain reaction analyses demonstrated GSP did not affect sterol regulatory element binding protein-2 and low-density lipoprotein receptor; however, it increased mRNA 3-hydroxy-3-methylglutaryl coenzyme A reductase. GSP had no effect on the protein mass of liver X receptor alpha (LXRα) but it decreased mRNA LXRα. Most importantly, GSP increased not only the protein level of cholesterol-7α-hydroxylase (CYP7A1) but also mRNA CYP7A1. It was concluded that the hypocholesterolemic activity of GSP was most likely mediated by enhancement of bile acid excretion and up-regulation of CYP7A1.
Authors:
Rui Jiao; Zesheng Zhang; Hongjian Yu; Yu Huang; Zhen-Yu Chen
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-01-25
Journal Detail:
Title:  The Journal of nutritional biochemistry     Volume:  21     ISSN:  1873-4847     ISO Abbreviation:  J. Nutr. Biochem.     Publication Date:  2010 Nov 
Date Detail:
Created Date:  2010-10-25     Completed Date:  2011-01-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9010081     Medline TA:  J Nutr Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1134-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2010 Elsevier Inc. All rights reserved.
Affiliation:
Food and Nutritional Sciences Programme, Department of Biochemistry, The Chinese University of Hong Kong, Shatin, NT, Hong Kong, China.
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MeSH Terms
Descriptor/Qualifier:
Animals
Bile Acids and Salts / genetics,  metabolism*
Cholesterol / blood*,  metabolism
Cholesterol 7-alpha-Hydroxylase / genetics,  metabolism*
Cricetinae
Grape Seed Extract / pharmacology*
Male
Mesocricetus
Orphan Nuclear Receptors / metabolism
Proanthocyanidins / pharmacology*
Receptors, LDL / metabolism
Sterol Regulatory Element Binding Protein 2 / genetics,  metabolism
Up-Regulation
Chemical
Reg. No./Substance:
0/Bile Acids and Salts; 0/Grape Seed Extract; 0/Grape Seed Proanthocyanidins; 0/Orphan Nuclear Receptors; 0/Proanthocyanidins; 0/Receptors, LDL; 0/Sterol Regulatory Element Binding Protein 2; 0/liver X receptor; 57-88-5/Cholesterol; EC 1.14.13.17/Cholesterol 7-alpha-Hydroxylase

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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