| Hypocholesterolemic effects of hydroxypropyl methylcellulose are mediated by altered gene expression in hepatic bile and cholesterol pathways of male hamsters. | |
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MedLine Citation:
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PMID: 20444951 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hydroxypropyl methylcellulose (HPMC), a semisynthetic, nonfermentable soluble dietary fiber, is not absorbed by the body, but its presence in the intestinal lumen increases fecal fat, sterol, and bile acid excretions and decreases intestinal cholesterol absorption, all of which may indirectly affect hepatic lipid metabolism. We measured the expression of hepatic genes involved in cholesterol, bile acid, and fatty acid metabolism in hamsters fed diets containing 39% of energy as fat and 5% of weight as HPMC or microcrystalline cellulose (control) for 4 wk. HPMC-fed hamsters gained significantly less body weight than the control group but did not differ in food intake. They had significantly lower plasma triglyceride and total-, VLDL-, HDL-, and LDL-cholesterol concentrations and hepatic total lipid, total and free cholesterol and triglyceride concentrations than controls. Compared with controls, HPMC-fed hamsters had greater levels of mRNA for CYP7A1 (cytochrome P450 7A1; 8-fold of control; P < 0.05), CYP51 (lanosterol 14alpha-demethylase; 5.3-fold of control; P < 0.05), and HMG-CoAR (3-hydroxy-3-methylglutaryl CoA reductase; 1.8-fold of control; P < 0.05). The plasma total cholesterol concentrations from both the control and HPMC groups were inversely correlated with expression of hepatic CYP7A1 (r = -0.54; P < 0.05), CYP51 (r = -0.79; P < 0.005), and HMG-CoAR (r = -0.75; P < 0.005) genes. This suggests that HPMC supplementation affected both cholesterol and bile acid synthesis. Our data confirm that altered hepatic expression of lipid metabolism-related genes, possibly due to modulation of fecal bile acid excretion and intestinal cholesterol absorption, contributes to the lipid-lowering effects of HPMC. |
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Authors:
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Glenn E Bartley; Wallace Yokoyama; Scott A Young; William H K Anderson; Shao-Ching Hung; David R Albers; Marsha L Langhorst; Hyunsook Kim |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-05-05 |
Journal Detail:
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Title: The Journal of nutrition Volume: 140 ISSN: 1541-6100 ISO Abbreviation: J. Nutr. Publication Date: 2010 Jul |
Date Detail:
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Created Date: 2010-06-21 Completed Date: 2010-07-21 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 0404243 Medline TA: J Nutr Country: United States |
Other Details:
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Languages: eng Pagination: 1255-60 Citation Subset: IM |
Affiliation:
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Processed Foods Research, Western Regional Research Center, USDA, Agricultural Research Service, Albany, CA 94710, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adiponectin
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blood Animals Anticholesteremic Agents / pharmacology* Bile / metabolism* Blood Glucose / analysis Cholesterol / metabolism* Cricetinae Gene Expression / drug effects* Insulin / blood Liver / drug effects*, metabolism Male Mesocricetus Methylcellulose / analogs & derivatives*, pharmacology Polymerase Chain Reaction RNA, Messenger / genetics |
| Chemical | |
Reg. No./Substance:
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0/Adiponectin; 0/Anticholesteremic Agents; 0/Blood Glucose; 0/RNA, Messenger; 11061-68-0/Insulin; 57-88-5/Cholesterol; 8063-82-9/hypromellose; 9004-67-5/Methylcellulose |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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