| Hypochlorous acid potentiates hydrogen peroxide-mediated DNA-strand breaks in human mononuclear leucocytes. | |
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MedLine Citation:
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PMID: 1370724 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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In this study the formation of DNA single-strand breaks in MNL in close proximity to activated phagocytes, or in contact with added H2O2 and/or HOCl, were evaluated. Neutrophils activated by phorbol myristate acetate (PMA), induced DNA-strand breaks in neighboring lymphocytes which increased after 1-2 h incubation in a repair medium. These DNA-strand breaks could be prevented by the addition of catalase or substitution of the neutrophils with cells from a patient with chronic granulomatous disease. Inclusion of the myeloperoxidase (MPO) inhibitor, sodium azide (NaN3), to the system was associated with less damage after 1-2 h incubation and a faster repair rate. Exposure of MNL to added reagent H2O2 (12-100 microM) was also accompanied by DNA damage. Addition of reagent HOCl (3-25 microM) did not induce any DNA-strand breaks. However, when combined with H2O2 (12.5 microM), HOCl increased H2O2-mediated DNA damage and compromised the repair process. Interactions between the phagocyte-derived reactive oxidants H2O2 and HOCl are probably involved in the etiology of inflammation-related cancer. |
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Authors:
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C E Van Rensburg; A M Van Staden; R Anderson; E J Van Rensburg |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Mutation research Volume: 265 ISSN: 0027-5107 ISO Abbreviation: Mutat. Res. Publication Date: 1992 Feb |
Date Detail:
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Created Date: 1992-02-27 Completed Date: 1992-02-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0400763 Medline TA: Mutat Res Country: NETHERLANDS |
Other Details:
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Languages: eng Pagination: 255-61 Citation Subset: IM |
Affiliation:
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Department of Immunology, Faculty of Medicine, University of Pretoria, Republic of South Africa. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Adult DNA Damage* Drug Synergism Humans Hydrogen Peroxide / toxicity* Hypochlorous Acid / pharmacology* Kinetics Leukocytes, Mononuclear / drug effects* Mutagenicity Tests Neutrophils / immunology |
| Chemical | |
Reg. No./Substance:
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7722-84-1/Hydrogen Peroxide; 7790-92-3/Hypochlorous Acid |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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