| Hypervariable loci that enhance the discriminatory ability of newly proposed 15-loci and 24-loci variable-number tandem repeat typing method on Mycobacterium tuberculosis strains predominated by the Beijing family. | |
| | |
MedLine Citation:
|
PMID: 17302938 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The newly proposed 15- and 24-loci mycobacterial interspersed repetitive unit (MIRU)-variable-number tandem repeat (VNTR) typing method was evaluated for its ability to differentiate 181 Mycobacterium tuberculosis Beijing family strains. Compared with the original 12-loci MIRU-VNTR typing method, the 15-loci system dramatically improved the discriminatory power for Beijing strains; however, large clusters that could be further differentiated by IS6110 restriction fragment length polymorphism (RFLP) were still obtained. The clonal stability and allelic diversity of a total of 31 VNTR loci were evaluated. VNTRs 3232, 3820, and 4120 were identified as the effective hypervariable VNTR set for the second-line typing of clustered strains following the 15-loci based scheme. Consequently, the discriminatory power of the new scheme (18 loci) equaled that of IS6110 RFLP. |
| | |
Authors:
|
Tomotada Iwamoto; Shiomi Yoshida; Katsuhiro Suzuki; Motohisa Tomita; Riyo Fujiyama; Noriko Tanaka; Yasuto Kawakami; Masahiro Ito |
Related Documents
:
|
10078598 - Rapid detection of mycobacterium bovis on its lipid profile by thin layer chromatography. 1420348 - A new glycolipid from mycobacterium avium--mycobacterium intracellulare complex. 10439408 - A novel polymorphic genetic locus in members of the mycobacterium tuberculosis complex. 3771768 - Mycolic acid analysis for clinical identification of mycobacterium avium and related my... 18783898 - The effect of rehabilitation of northern elephant seals (mirounga angustirostris) on an... 374678 - The isolation and characterization of three types of vitamin b6 auxotrophs of escherich... |
Publication Detail:
|
Type: Journal Article Date: 2007-02-16 |
Journal Detail:
|
Title: FEMS microbiology letters Volume: 270 ISSN: 0378-1097 ISO Abbreviation: FEMS Microbiol. Lett. Publication Date: 2007 May |
Date Detail:
|
Created Date: 2007-04-18 Completed Date: 2007-08-13 Revised Date: 2007-08-15 |
Medline Journal Info:
|
Nlm Unique ID: 7705721 Medline TA: FEMS Microbiol Lett Country: England |
Other Details:
|
Languages: eng Pagination: 67-74 Citation Subset: IM |
Affiliation:
|
Department of Microbiology, Kobe Institute of Health, Kobe, Japan. kx2t-iwmt@asahi-net.or.jp |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Bacterial Typing Techniques China Family Health Humans Interspersed Repetitive Sequences / genetics* Minisatellite Repeats / genetics* Mycobacterium tuberculosis / classification, genetics*, isolation & purification Polymerase Chain Reaction Polymorphism, Restriction Fragment Length Tuberculosis / microbiology |
| Comments/Corrections | |
Erratum In:
|
FEMS Microbiol Lett. 2007 Jul;272(2):282-3 |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Inter- and intraspecific genomic variability of the 16S-23S intergenic spacer regions (ISR) in repre...
Next Document: Involvement of Rho-type GTPase in control of cell size in Saccharomyces cerevisiae.