| Hyperuricemia, oxidative stress, and carotid artery tone in experimental renal insufficiency. | |
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MedLine Citation:
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PMID: 19521342 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Hyperuricemia may play a role in the pathogenesis of cardiovascular disease, but uric acid is also a significant antioxidant. We investigated the effects of oxonic acid-induced hyperuricemia on carotid artery tone in experimental renal insufficiency. METHODS: Three weeks after 5/6 nephrectomy (NX) or Sham operation, male Sprague-Dawley rats were allocated to 2.0% oxonic acid or control diet for 9 weeks. Blood pressure was monitored using tail cuff, isolated arterial rings were examined using myographs, and blood and urine samples were taken, as appropriate. Oxidative stress and antioxidant status were evaluated by measuring urinary 8-isoprostaglandin F(2 alpha) (8-iso-PGF(2 alpha)) excretion and plasma total peroxyl radical-trapping capacity (TRAP), respectively. RESULTS: Plasma creatinine was elevated twofold in NX rats, but neither NX nor oxonic acid diet influenced blood pressure. Urinary 8-iso-PGF(2 alpha) excretion was increased over 2.5-fold in NX rats on control diet. Oxonic acid diet increased plasma uric acid 2-3-fold, TRAP 1.5-fold, and reduced urinary 8-iso-PGF(2 alpha) excretion by 60-90%. Carotid vasorelaxation to acetylcholine in vitro, which could be abolished by nitric oxide (NO) synthase inhibition, was reduced following NX, whereas maximal response to acetylcholine was augmented in hyperuricemic NX rats. Vasorelaxation to nitroprusside was impaired in NX rats, whereas oxonic acid diet increased sensitivity also to nitroprusside in NX rats. CONCLUSIONS: Oxonic acid-induced hyperuricemia reduced oxidative stress in vivo, as evaluated using urinary 8-iso-PGF(2 alpha) excretion, increased plasma TRAP, and improved NO-mediated vasorelaxation in the carotid artery in experimental renal insufficiency. |
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Authors:
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Venla Kurra; Arttu Eräranta; Pasi Jolma; Tuija I Vehmas; Asko Riutta; Eeva Moilanen; Anna Tahvanainen; Jarkko Kalliovalkama; Onni Niemelä; Juhani Myllymäki; Jukka Mustonen; Ilkka Pörsti |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2009-06-11 |
Journal Detail:
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Title: American journal of hypertension Volume: 22 ISSN: 1941-7225 ISO Abbreviation: Am. J. Hypertens. Publication Date: 2009 Sep |
Date Detail:
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Created Date: 2009-08-24 Completed Date: 2009-12-03 Revised Date: 2011-06-30 |
Medline Journal Info:
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Nlm Unique ID: 8803676 Medline TA: Am J Hypertens Country: United States |
Other Details:
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Languages: eng Pagination: 964-70 Citation Subset: IM |
Affiliation:
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Medical School, University of Tampere, Tampere, Finland. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Carotid Arteries / drug effects, physiology Creatinine / blood Dinoprost / analogs & derivatives, urine Hyperuricemia / chemically induced, physiopathology* Male NG-Nitroarginine Methyl Ester / pharmacology Nephrectomy Oxidative Stress / drug effects* Oxonic Acid Peroxides / metabolism Rats Rats, Sprague-Dawley Renal Insufficiency / physiopathology* Uric Acid / blood Vasodilation / drug effects |
| Chemical | |
Reg. No./Substance:
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0/Peroxides; 27415-26-5/8-epi-prostaglandin F2alpha; 3170-83-0/perhydroxyl radical; 50903-99-6/NG-Nitroarginine Methyl Ester; 551-11-1/Dinoprost; 60-27-5/Creatinine; 69-93-2/Uric Acid; 937-13-3/Oxonic Acid |
| Comments/Corrections | |
Comment In:
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Am J Hypertens. 2009 Sep;22(9):952-3
[PMID:
19701166
]
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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