Document Detail

Hyperuricemia, oxidative stress, and carotid artery tone in experimental renal insufficiency.
MedLine Citation:
PMID:  19521342     Owner:  NLM     Status:  MEDLINE    
BACKGROUND: Hyperuricemia may play a role in the pathogenesis of cardiovascular disease, but uric acid is also a significant antioxidant. We investigated the effects of oxonic acid-induced hyperuricemia on carotid artery tone in experimental renal insufficiency.
METHODS: Three weeks after 5/6 nephrectomy (NX) or Sham operation, male Sprague-Dawley rats were allocated to 2.0% oxonic acid or control diet for 9 weeks. Blood pressure was monitored using tail cuff, isolated arterial rings were examined using myographs, and blood and urine samples were taken, as appropriate. Oxidative stress and antioxidant status were evaluated by measuring urinary 8-isoprostaglandin F(2 alpha) (8-iso-PGF(2 alpha)) excretion and plasma total peroxyl radical-trapping capacity (TRAP), respectively.
RESULTS: Plasma creatinine was elevated twofold in NX rats, but neither NX nor oxonic acid diet influenced blood pressure. Urinary 8-iso-PGF(2 alpha) excretion was increased over 2.5-fold in NX rats on control diet. Oxonic acid diet increased plasma uric acid 2-3-fold, TRAP 1.5-fold, and reduced urinary 8-iso-PGF(2 alpha) excretion by 60-90%. Carotid vasorelaxation to acetylcholine in vitro, which could be abolished by nitric oxide (NO) synthase inhibition, was reduced following NX, whereas maximal response to acetylcholine was augmented in hyperuricemic NX rats. Vasorelaxation to nitroprusside was impaired in NX rats, whereas oxonic acid diet increased sensitivity also to nitroprusside in NX rats.
CONCLUSIONS: Oxonic acid-induced hyperuricemia reduced oxidative stress in vivo, as evaluated using urinary 8-iso-PGF(2 alpha) excretion, increased plasma TRAP, and improved NO-mediated vasorelaxation in the carotid artery in experimental renal insufficiency.
Venla Kurra; Arttu Eräranta; Pasi Jolma; Tuija I Vehmas; Asko Riutta; Eeva Moilanen; Anna Tahvanainen; Jarkko Kalliovalkama; Onni Niemelä; Juhani Myllymäki; Jukka Mustonen; Ilkka Pörsti
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2009-06-11
Journal Detail:
Title:  American journal of hypertension     Volume:  22     ISSN:  1941-7225     ISO Abbreviation:  Am. J. Hypertens.     Publication Date:  2009 Sep 
Date Detail:
Created Date:  2009-08-24     Completed Date:  2009-12-03     Revised Date:  2011-06-30    
Medline Journal Info:
Nlm Unique ID:  8803676     Medline TA:  Am J Hypertens     Country:  United States    
Other Details:
Languages:  eng     Pagination:  964-70     Citation Subset:  IM    
Medical School, University of Tampere, Tampere, Finland.
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MeSH Terms
Carotid Arteries / drug effects,  physiology
Creatinine / blood
Dinoprost / analogs & derivatives,  urine
Hyperuricemia / chemically induced,  physiopathology*
NG-Nitroarginine Methyl Ester / pharmacology
Oxidative Stress / drug effects*
Oxonic Acid
Peroxides / metabolism
Rats, Sprague-Dawley
Renal Insufficiency / physiopathology*
Uric Acid / blood
Vasodilation / drug effects
Reg. No./Substance:
0/Peroxides; 27415-26-5/8-epi-prostaglandin F2alpha; 3170-83-0/perhydroxyl radical; 50903-99-6/NG-Nitroarginine Methyl Ester; 551-11-1/Dinoprost; 60-27-5/Creatinine; 69-93-2/Uric Acid; 937-13-3/Oxonic Acid
Comment In:
Am J Hypertens. 2009 Sep;22(9):952-3   [PMID:  19701166 ]

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