Document Detail

Hypertrophic versus non hypertrophic scars compared by immunohistochemistry and laser confocal microscopy: type I and III collagens.
MedLine Citation:
PMID:  20051096     Owner:  NLM     Status:  MEDLINE    
Although dermal collagens appear increased in hypertrophic scars, this has not been tested in tissue samples using objective methods. We compared the expression of types I and III collagen in hypertrophic and non hypertrophic scars at 6-12 and 18-24 months after burn using a quantitative method. Among 17 patients with extensive burns, 3 patients had acute scars, 8 had hypertrophic or non hypertrophic scars at 6-12 months after burn and 6 had hypertrophic or non hypertrophic scars at 18-24 months after burn. After clinical assessment of scars using the Vancouver scale, immunohistochemistry for types I and III collagens was performed. Images were captured with a laser scanning confocal microscope and the relative amounts of types I and III collagens were determined in superficial and deep dermis. The effects of time and scar type were assessed using two-way analysis of variance (ANOVA) and Tukey's test. Collagen III scar/normal ratios were higher in hypertrophic scars at both time points (P = 0.05). There were no differences in collagen I scar/normal ratios. Large variation was observed in scars during the acute phase regarding the expression of collagens. Easily accessed by immunohistochemistry and confocal microscopy, type III collagen deposition may help in determining scar phenotype, differentiating hypertrophic and non hypertrophic scars.
Gisele V Oliveira; Hal K Hawkins; David Chinkes; Ann Burke; Andre Luiz Pasqua Tavares; Marcia Ramos-e-Silva; Thomas B Albrecht; Gregory T Kitten; David N Herndon
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Publication Detail:
Type:  Comparative Study; Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  International wound journal     Volume:  6     ISSN:  1742-481X     ISO Abbreviation:  Int Wound J     Publication Date:  2009 Dec 
Date Detail:
Created Date:  2010-01-06     Completed Date:  2010-03-18     Revised Date:  2014-05-23    
Medline Journal Info:
Nlm Unique ID:  101230907     Medline TA:  Int Wound J     Country:  England    
Other Details:
Languages:  eng     Pagination:  445-52     Citation Subset:  IM    
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MeSH Terms
Burns / complications,  metabolism,  pathology
Child, Preschool
Cicatrix, Hypertrophic / etiology,  metabolism,  pathology*
Collagen Type I / analysis*
Collagen Type III / analysis*
Follow-Up Studies
Immunohistochemistry / methods*
Microscopy, Confocal / methods*
Skin / injuries,  metabolism,  pathology
Trauma Severity Indices
Wound Healing / physiology*
Grant Support
60338//PHS HHS; H133A970019//PHS HHS; M01 RR000073/RR/NCRR NIH HHS; P50 GM060338/GM/NIGMS NIH HHS
Reg. No./Substance:
0/Collagen Type I; 0/Collagen Type III

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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