Document Detail

Hypertriglyceridemia and cholesteryl ester transfer protein interact to dramatically alter high density lipoprotein levels, particle sizes, and metabolism. Studies in transgenic mice.
MedLine Citation:
PMID:  8376576     Owner:  NLM     Status:  MEDLINE    
Several types of transgenic mice were used to study the influence of hypertriglyceridemia and cholesteryl ester transfer protein (CETP) expression on high density lipoprotein (HDL) levels, particle sizes, and metabolism. The presence of the CETP transgene in hypertriglyceridemic human apo CIII transgenic mice lowered HDL-cholesterol (HDL-C) 48% and apolipoprotein (apo) A-I 40%, decreased HDL size (particle diameter from 9.8 to 8.8 nm), increased HDL cholesterol ester (CE) fractional catabolic rate (FCR) 65% with a small decrease in HDL CE transport rate (TR) and increased apo A-I FCR 15% and decreased apo A-I TR 29%. The presence of the CETP transgene in hypertriglyceridemic mice with human-like HDL, human apo A-I apo CIII transgenic mice, lowered HDL-C 61% and apo A-I 45%, caused a dramatic diminution of HDL particle size (particle diameters from 10.3 and 9.1 to 7.6 nm), increased HDL CE FCR by 107% without affecting HDL CE TR, and increased apo A-I FCR 35% and decreased apo A-I TR 48%. Moreover, unexpectedly, hypertriglyceridemia alone in the absence of CETP was also found to cause lower HDL-C and apo A-I levels primarily by decreasing TRs. Decreased apo A-I TR was confirmed by an in vivo labeling study and found to be associated with a decrease in intestinal but not hepatic apo A-I mRNA levels. In summary, the introduction of the human apo A-I, apo CIII, and CETP genes into transgenic mice produced a high-triglyceride, low-HDL-C lipoprotein phenotype. Human apo A-I gene overexpression caused a diminution of mouse apo A-I and a change from monodisperse to polydisperse HDL. Human apo CIII gene overexpression caused hypertriglyceridemia with a significant decrease in HDL-C and apo A-I levels primarily due to decreased HDL CE and apo A-I TR but without a profound change in HDL size. In the hypertriglyceridemic mice, human CETP gene expression further reduced HDL-C and apo A-I levels, primarily by increasing HDL CE and apo A-I FCR, while dramatically reducing HDL size. This study provides insights into the genes that may cause the high-triglyceride, low-HDL-C phenotype in humans and the metabolic mechanisms involved.
T Hayek; N Azrolan; R B Verdery; A Walsh; T Chajek-Shaul; L B Agellon; A R Tall; J L Breslow
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  The Journal of clinical investigation     Volume:  92     ISSN:  0021-9738     ISO Abbreviation:  J. Clin. Invest.     Publication Date:  1993 Sep 
Date Detail:
Created Date:  1993-10-15     Completed Date:  1993-10-15     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7802877     Medline TA:  J Clin Invest     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1143-52     Citation Subset:  AIM; IM    
Laboratory of Biochemical Genetics and Metabolism, Rockefeller University, New York 10021-6399.
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MeSH Terms
Apolipoprotein A-I / genetics
Apolipoproteins / metabolism
Carrier Proteins / metabolism*
Cholesterol Ester Transfer Proteins
Gene Expression
Hypertriglyceridemia / metabolism*
Lipoproteins, HDL / metabolism*
Mice, Transgenic
RNA, Messenger / genetics
Triglycerides / metabolism
Grant Support
Reg. No./Substance:
0/Apolipoprotein A-I; 0/Apolipoproteins; 0/CETP protein, human; 0/Carrier Proteins; 0/Cholesterol Ester Transfer Proteins; 0/Glycoproteins; 0/Lipoproteins, HDL; 0/RNA, Messenger; 0/Triglycerides

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