Document Detail

Hypertonic stress regulates amino acid transport and cell cycle proteins in chick embryo hepatocytes.
MedLine Citation:
PMID:  21906028     Owner:  NLM     Status:  MEDLINE    
Hyperosmotic stress affects cell growth, decreasing cell volume and increasing the uptake of organic osmolytes. However, the sensitivity of embryonic cells to osmotic treatment remains to be established. We have analysed some aspects of cell-cycle control and amino-acid transport in hypertonic conditions during prenatal life. The effects of hyperosmotic stress on amino-acid uptake mediated by system A, (3)H-thymidine incorporation, and regulation of cell-cycle proteins were analysed in chick embryo hepatocytes. Hypertonic stress increased system A activity and caused cell-cycle delay. Effects on amino-acid transport involved p38 kinase activation and new carrier synthesis. Cyclin D1, cdk4 (cyclin-dependent kinase 4) and PCNA (proliferating-cell nuclear antigen) levels decreased, whereas cyclin E, p21 and p53 levels were unchanged. Incorporation of (3)H-leucine indicated decreased synthesis of cyclin D1. In contrast, analysis of mRNA by qRT-PCR (quantitative real-time PCR) showed a net increase of cyclin D1 transcripts, suggesting post-transcriptional regulation. The data show that chick embryo hepatocytes respond to hyperosmotic conditions by arresting cell growth to prevent DNA damage and increasing osmolyte uptake to regulate cell volume, indicating that the adaptive response to environmental stress exists during prenatal life.
Giovannella Bruscalupi; Mara Massimi; Silvana Spagnuolo; Anna Maria Fiore; Silvia Leoni
Related Documents :
17373578 - The identification of two germ-line mutations in the human breast cancer resistance pro...
2311168 - Metallothionein-like proteins and cell resistance to cis-dichlorodiammineplatinum(ii) i...
226998 - Toad urinary bladder epithelial cells in culture: maintenance of epithelial structure, ...
6251098 - Control and virus-transformed baby hamster kidney cells resistant to ethidium bromide. ...
21670168 - Analysis of the hd-gyp domain cyclic dimeric gmp phosphodiesterase reveals a role in mo...
18235008 - Response of human trabecular meshwork cells to topographic cues on the nanoscale level.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Cell biology international     Volume:  36     ISSN:  1095-8355     ISO Abbreviation:  Cell Biol. Int.     Publication Date:  2012 Feb 
Date Detail:
Created Date:  2012-01-06     Completed Date:  2012-04-25     Revised Date:  2012-08-02    
Medline Journal Info:
Nlm Unique ID:  9307129     Medline TA:  Cell Biol Int     Country:  England    
Other Details:
Languages:  eng     Pagination:  203-13     Citation Subset:  IM    
Department of Biology and Biotechnology 'Charles Darwin', Sapienza University of Rome, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Amino Acids / metabolism*
Biological Transport
Cell Cycle Proteins / metabolism*
Cell Size
Cells, Cultured
Chick Embryo
Cyclin D1 / genetics,  metabolism
Cyclin E / metabolism
Cyclin-Dependent Kinase 4 / metabolism
Cyclin-Dependent Kinase Inhibitor p21 / genetics,  metabolism
Hepatocytes / drug effects*,  metabolism
Hypertonic Solutions / pharmacology*
Mitogen-Activated Protein Kinase 1 / antagonists & inhibitors,  metabolism
Mitogen-Activated Protein Kinase 3 / antagonists & inhibitors,  metabolism
Proliferating Cell Nuclear Antigen / metabolism
Stress, Physiological*
Tumor Suppressor Protein p53 / metabolism
p38 Mitogen-Activated Protein Kinases / antagonists & inhibitors,  metabolism
Reg. No./Substance:
0/Amino Acids; 0/Cell Cycle Proteins; 0/Cyclin E; 0/Cyclin-Dependent Kinase Inhibitor p21; 0/Hypertonic Solutions; 0/Proliferating Cell Nuclear Antigen; 0/Tumor Suppressor Protein p53; 136601-57-5/Cyclin D1; EC Kinase 4; EC Protein Kinase 1; EC Protein Kinase 3; EC Mitogen-Activated Protein Kinases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  S6K inhibition renders cardiac protection against myocardial infarction through PDK1 phosphorylation...
Next Document:  Neurohumoral and haemodynamic profile in postural tachycardia and chronic fatigue syndromes.