| Hypertonic saline resuscitation attenuates neutrophil lung sequestration and transmigration by diminishing leukocyte-endothelial interactions in a two-hit model of hemorrhagic shock and infection. | |
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MedLine Citation:
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PMID: 12544907 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Hypertonic saline (HTS) attenuates polymorphonuclear neutrophil (PMN)-mediated tissue injury after hemorrhagic shock. We hypothesized that HTS resuscitation reduces early in vivo endothelial cell (EC)-PMN interactions and late lung PMN sequestration in a two-hit model of hemorrhagic shock followed by mimicked infection. METHODS: Thirty-two mice were hemorrhaged (40 mm Hg) for 60 minutes and then given intratracheal lipopolysaccharide (10 microg) 1 hour after resuscitation with shed blood and either HTS (4 mL/kg 7.5% NaCl) or Ringer's lactate (RL) (twice shed blood volume). Eleven controls were not manipulated. Cremaster intravital microscopy quantified 5-hour EC-PMN adherence, myeloperoxidase assay assessed lung PMN content (2 1/2 and 24 hours), and lung histology determined 24-hour PMN transmigration. RESULTS: Compared with RL, HTS animals displayed 55% less 5-hour EC-PMN adherence (p = 0.01), 61% lower 24-hour lung myeloperoxidase ( p= 0.007), and 57% lower mean 24-hour lung histologic score ( p= 0.027). CONCLUSION: Compared with RL, HTS resuscitation attenuates early EC-PMN adhesion and late lung PMN accumulation in hemorrhagic shock followed by inflammation. HTS resuscitation may attenuate PMN-mediated organ damage. |
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Authors:
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José L Pascual; Kosar A Khwaja; Lorenzo E Ferri; Betty Giannias; David C Evans; Tarek Razek; René P Michel; Nicolas V Christou |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: The Journal of trauma Volume: 54 ISSN: 0022-5282 ISO Abbreviation: J Trauma Publication Date: 2003 Jan |
Date Detail:
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Created Date: 2003-01-24 Completed Date: 2003-02-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0376373 Medline TA: J Trauma Country: United States |
Other Details:
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Languages: eng Pagination: 121-30; discussion 130-2 Citation Subset: AIM; IM |
Affiliation:
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Division of General Surgery, McGill University Health Center, Montréal, Québec, Canada. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Disease Models, Animal* Drug Evaluation, Preclinical Endothelium, Vascular / drug effects, immunology* Escherichia coli Fluid Therapy / methods* Inflammation Isotonic Solutions / pharmacology, therapeutic use Lipopolysaccharides / adverse effects Male Mice Mice, Inbred Strains Neutrophil Infiltration / immunology* Neutrophils / drug effects, immunology* Peroxidase / analysis Random Allocation Respiratory Distress Syndrome, Adult / enzymology, etiology*, pathology, prevention & control Resuscitation / methods* Saline Solution, Hypertonic / pharmacology, therapeutic use* Sepsis / complications*, drug therapy*, immunology Shock, Hemorrhagic / complications*, drug therapy*, immunology Single-Blind Method Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Isotonic Solutions; 0/Lipopolysaccharides; 0/Saline Solution, Hypertonic; 8022-63-7/Ringer's lactate; EC 1.11.1.7/Peroxidase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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