Document Detail

Hypertonic saline nasal provocation stimulates nociceptive nerves, substance P release, and glandular mucous exocytosis in normal humans.
MedLine Citation:
PMID:  10430743     Owner:  NLM     Status:  MEDLINE    
Hypertonic saline (HTS) induces bronchoconstriction. Potential mechanisms were evaluated in a human nasal provocation model. Aliquots of normal saline (1 x NS, 100 microliters) and higher concentrations (3 x NS, 6 x NS, 12 x NS, 24 x NS) were sprayed into one nostril at 5-min intervals. Lavage fluids were collected from the ipsilateral and contralateral sides to determine the concentrations of specific mucus constituents. Nasal cavity air-space volume was assessed by acoustic rhinometry (AcRh). The distribution of substance-P-preferring neurokinin-1 (NK-1) receptor mRNA was assessed by in situ reverse transcriptase-polymerase chain reaction. Unilateral HTS induced unilateral dose-dependent increases in sensations of pain, blockage, and rhinorrhea, the weights of recovered lavage fluids, and concentrations of total protein, lactoferrin, mucoglycoprotein markers, and substance P. Contralateral, reflex-mediated effects were minor. There were no changes in IgG or AcRh measurements. NK-1 receptor mRNA was localized to submucosal glands. HTS caused pain with unilateral substance P release. The presumed nociceptive nerve efferent axon response led to glandular exocytosis, presumably through actions on submucosal gland NK-1 receptors. Vascular processes, including plasma exudation, filling of venous sinusoids, and mucosal edema were not induced in these normal subjects.
J N Baraniuk; M Ali; A Yuta; S Y Fang; K Naranch
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  American journal of respiratory and critical care medicine     Volume:  160     ISSN:  1073-449X     ISO Abbreviation:  Am. J. Respir. Crit. Care Med.     Publication Date:  1999 Aug 
Date Detail:
Created Date:  1999-08-30     Completed Date:  1999-08-30     Revised Date:  2007-11-14    
Medline Journal Info:
Nlm Unique ID:  9421642     Medline TA:  Am J Respir Crit Care Med     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  655-62     Citation Subset:  AIM; IM    
Division of Rheumatology, Immunology and Allergy, Georgetown University, Washington, DC, USA.
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MeSH Terms
Administration, Intranasal
Bronchial Provocation Tests*
Dose-Response Relationship, Drug
Exocytosis / drug effects*
Mucus / secretion*
Nasal Mucosa / drug effects*
Nociceptors / drug effects*
Receptors, Neurokinin-1 / drug effects
Reference Values
Reverse Transcriptase Polymerase Chain Reaction
Saline Solution, Hypertonic / diagnostic use*
Substance P / metabolism*
Grant Support
Reg. No./Substance:
0/Receptors, Neurokinin-1; 0/Saline Solution, Hypertonic; 33507-63-0/Substance P

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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