Document Detail

Hypertonic activation of a non-selective cation conductance in HeLa cells and its contribution to cell volume regulation.
MedLine Citation:
PMID:  12965198     Owner:  NLM     Status:  MEDLINE    
In whole-cell recordings on single HeLa cells, the hypertonic activation of a cation conductance with a selectivity ratio P(Na):P(Li):P(K):P(Cs):P(NMDG):P(Ca):P(Cl) of 1.00:0.86:0.84:0.56:0.10:0.07:0.15 was observed. This (non-selective) cation conductance was reduced to 59 and 30% of maximal stimulation by Gd(3+) and flufenamate, respectively, but it was insensitive to amiloride (with each compound applied at 100 microm/l). As was determined by the Coulter counter technique, the cation conductance was the main mechanism of regulatory volume increase (RVI) in HeLa cells. Whereas a significant contribution of Na(+)/H(+) antiport was also detectable, Na(+)-K(+)-2Cl(-) symport most likely did not contribute to RVI.
Frank Wehner; Takahiro Shimizu; Ravshan Sabirov; Yasunobu Okada
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  FEBS letters     Volume:  551     ISSN:  0014-5793     ISO Abbreviation:  FEBS Lett.     Publication Date:  2003 Sep 
Date Detail:
Created Date:  2003-09-10     Completed Date:  2003-11-05     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0155157     Medline TA:  FEBS Lett     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  20-4     Citation Subset:  IM    
Department of Cell Physiology, National Institute for Physiological Sciences, 444-8585 Okazaki, Japan.
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MeSH Terms
Cations / metabolism
Cell Size*
Electric Conductivity
Hela Cells
Ion Pumps / metabolism*
Osmolar Concentration
Patch-Clamp Techniques
Sodium-Hydrogen Antiporter / metabolism
Sodium-Potassium-Chloride Symporters / metabolism
Reg. No./Substance:
0/Cations; 0/Ion Pumps; 0/Sodium-Hydrogen Antiporter; 0/Sodium-Potassium-Chloride Symporters

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