| Hyperthyroidism in the developing rat testis is associated with oxidative stress and hyperphosphorylated vimentin accumulation. | |
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MedLine Citation:
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PMID: 17306450 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Hyperthyroidism was induced in rats and somatic indices and metabolic parameters were analyzed in testis. In addition, the morphological analysis evidenced testes maturation and intense protein synthesis and processing, supporting the enhancement in vimentin synthesis in hyperthyroid testis. Furthermore, vimentin phosphorylation was increased, indicating an accumulation of phosphorylated vimentin associated to the cytoskeleton, which could be a consequence of the extracellular-regulated kinase (ERK) activation regulating the cytoskeleton. Biomarkers of oxidative stress demonstrated an increased basal metabolic rate measured by tissue oxygen consumption, as well as, increased TBARS levels. In addition, the enzymatic and non-enzymatic antioxidant defences appeared to respond according to the augmented oxygen consumption. We observed decreased total glutathione levels, with enhancement of reduced glutathione, whereas most of the antioxidant enzyme activities were induced. Otherwise, superoxide dismutase activity was inhibited. These results support the idea that an increase in mitochondrial ROS generation, underlying cellular oxidative damage, is a side effect of hyperthyroid-induced biochemical changes by which rat testis increase their metabolic capacity. |
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Authors:
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Ariane Zamoner; Kátia Padilha Barreto; Danilo Wilhelm Filho; Fabíola Sell; Viviane Mara Woehl; Fátima Costa Rodrigues Guma; Fátima Regina Mena Barreto Silva; Regina Pessoa-Pureur |
Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2007-01-20 |
Journal Detail:
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Title: Molecular and cellular endocrinology Volume: 267 ISSN: 0303-7207 ISO Abbreviation: Mol. Cell. Endocrinol. Publication Date: 2007 Mar |
Date Detail:
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Created Date: 2007-03-05 Completed Date: 2007-06-20 Revised Date: 2009-11-19 |
Medline Journal Info:
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Nlm Unique ID: 7500844 Medline TA: Mol Cell Endocrinol Country: Ireland |
Other Details:
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Languages: eng Pagination: 116-26 Citation Subset: IM |
Affiliation:
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Departamento de Bioquímica, Instituto de Ciências Básicas da Saúde, UFRGS, Porto Alegre, RS, Brazil. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Body Weight Enzymes Extracellular Signal-Regulated MAP Kinases / metabolism Gene Expression Regulation Glutathione / metabolism Glycoprotein Hormones, alpha Subunit / blood Hyperthyroidism / chemically induced, pathology* Lipid Peroxidation Male Organ Size Oxidative Stress* Oxygen Consumption Phosphorylation RNA, Messenger / genetics, metabolism Rats Rats, Wistar Testis / cytology, growth & development*, ultrastructure Thyroid Hormones / blood Vimentin / genetics, metabolism* |
| Chemical | |
Reg. No./Substance:
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0/Enzymes; 0/Glycoprotein Hormones, alpha Subunit; 0/RNA, Messenger; 0/Thyroid Hormones; 0/Vimentin; 70-18-8/Glutathione; EC 2.7.11.24/Extracellular Signal-Regulated MAP Kinases |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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