Document Detail

Hyperthermia inhibits cell proliferation and induces apoptosis: relative signaling status of P53, S100A4, and Notch in heat sensitive and resistant cell lines.
MedLine Citation:
PMID:  17497677     Owner:  NLM     Status:  MEDLINE    
The effects of hyperthermia on the expression of p53, the apoptosis-associated genes Bax and Bcl-2, Notch and S100A4 have been studied in the HepG2 cell line and the HUT cell line derived from HepG2, adapted for growth in hyperthermic conditions. Hyperthermia inhibits cell proliferation and induces apoptosis. HepG2 and HUT cells differed in respect of anchorage to growth surface, degree of proliferation and apoptosis and expression of p53, Bax, Bcl-2, Notch, and S100A4 genes. The induction of apoptosis and the inhibition of cell proliferation occurred independently of p53, and independently also of involvement of the apoptosis family genes Bax and Bcl-2. We demonstrate novel and marked differences between transient heat shock and heat adaptation in respect of pathways of signaling and generation of phenotypic effects in vitro. Different signaling patterns have been identified here. Pathways of signaling by S100A4, by its interaction with and sequestration of p53, and by Notch also seem differentially operational in the induction of apoptosis, and both appear to be activated as alternative pathways in the context of hyperthermia signaling independently of p53.
Antonio Basile; Daniela Biziato; Gajanan V Sherbet; Paola Comi; Francesco Cajone
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  103     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-24     Completed Date:  2008-02-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  212-20     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2007 Wiley-Liss, Inc.
Department of Biomedical Science and Technology, University of Milano, Milan, Italy.
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MeSH Terms
Cell Line, Tumor
Cell Proliferation
Gene Expression Regulation, Neoplastic
Heat-Shock Response*
Neoplasms / genetics,  metabolism,  pathology
Receptor, Notch1 / genetics,  metabolism*
S100 Proteins / metabolism*
Signal Transduction*
Tumor Suppressor Protein p53 / genetics,  metabolism*
Reg. No./Substance:
0/NOTCH1 protein, human; 0/Receptor, Notch1; 0/S100 Proteins; 0/Tumor Suppressor Protein p53; 142662-27-9/S100A4 protein, human

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