Document Detail


Hyperthermia inhibits cell proliferation and induces apoptosis: relative signaling status of P53, S100A4, and Notch in heat sensitive and resistant cell lines.
MedLine Citation:
PMID:  17497677     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The effects of hyperthermia on the expression of p53, the apoptosis-associated genes Bax and Bcl-2, Notch and S100A4 have been studied in the HepG2 cell line and the HUT cell line derived from HepG2, adapted for growth in hyperthermic conditions. Hyperthermia inhibits cell proliferation and induces apoptosis. HepG2 and HUT cells differed in respect of anchorage to growth surface, degree of proliferation and apoptosis and expression of p53, Bax, Bcl-2, Notch, and S100A4 genes. The induction of apoptosis and the inhibition of cell proliferation occurred independently of p53, and independently also of involvement of the apoptosis family genes Bax and Bcl-2. We demonstrate novel and marked differences between transient heat shock and heat adaptation in respect of pathways of signaling and generation of phenotypic effects in vitro. Different signaling patterns have been identified here. Pathways of signaling by S100A4, by its interaction with and sequestration of p53, and by Notch also seem differentially operational in the induction of apoptosis, and both appear to be activated as alternative pathways in the context of hyperthermia signaling independently of p53.
Authors:
Antonio Basile; Daniela Biziato; Gajanan V Sherbet; Paola Comi; Francesco Cajone
Related Documents :
16510557 - Mechanisms of induction of cell cycle arrest and cell death by cryptolepine in human lu...
9458377 - P53 modulation of fas/apo-1 mediated apoptosis in a human renal cell carcinoma cell line.
17497677 - Hyperthermia inhibits cell proliferation and induces apoptosis: relative signaling stat...
8959327 - Cell cycle-independent regulation of p21waf1/cip1 and retinoblastoma protein during oka...
21497547 - Use of a novel microfluidic disk in the analysis of single-cell viability and the appli...
12861887 - Effects of implant microtopography on osteoblast cell attachment.
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of cellular biochemistry     Volume:  103     ISSN:  0730-2312     ISO Abbreviation:  J. Cell. Biochem.     Publication Date:  2008 Jan 
Date Detail:
Created Date:  2007-12-24     Completed Date:  2008-02-29     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8205768     Medline TA:  J Cell Biochem     Country:  United States    
Other Details:
Languages:  eng     Pagination:  212-20     Citation Subset:  IM    
Copyright Information:
Copyright (c) 2007 Wiley-Liss, Inc.
Affiliation:
Department of Biomedical Science and Technology, University of Milano, Milan, Italy.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:
Apoptosis*
Cell Line, Tumor
Cell Proliferation
Gene Expression Regulation, Neoplastic
Heat-Shock Response*
Humans
Neoplasms / genetics,  metabolism,  pathology
Receptor, Notch1 / genetics,  metabolism*
S100 Proteins / metabolism*
Signal Transduction*
Tumor Suppressor Protein p53 / genetics,  metabolism*
Chemical
Reg. No./Substance:
0/NOTCH1 protein, human; 0/Receptor, Notch1; 0/S100 Proteins; 0/Tumor Suppressor Protein p53; 142662-27-9/S100A4 protein, human

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  The use of conformation-specific ligands and assays to dissect the molecular mechanisms of neurodege...
Next Document:  Runx2 determines bone maturity and turnover rate in postnatal bone development and is involved in bo...