| Hyperthermia-enhanced splenic cytokine gene expression is mediated by the sympathetic nervous system. | |
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MedLine Citation:
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PMID: 15292487 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Whole body hyperthermia (WBH) has been used in experimental settings as an adjunct to radiochemotherapy for the treatment of various malignant diseases. The therapeutic effect of WBH has been hypothesized to involve activation of the immune system, although the effect of hyperthermia-induced activation of sympathetic nerve discharge (SND) on splenic immune function is not known. We tested the hypothesis that heating-induced splenic sympathoexcitation would alter splenic cytokine gene expression as determined using gene array and real-time RT-PCR analyses. Experiments were performed in splenic-intact and splenic-denervated anesthetized Sprague-Dawley rats (n=32). Splenic SND was increased during heating (internal temperature increased from 38 degrees to 41 degrees C) in splenic-intact rats but remained unchanged in nonheated splenic-intact rats. Splenic interleukin-1beta (IL-1beta), interleukin-6 (IL-6), and growth-regulated oncogene 1 (GRO 1) mRNA expression was higher in heated than in nonheated splenic-intact rats. Splenic IL-1beta, IL-6, and GRO 1 mRNA expression was reduced in heated splenic-denervated compared with heated splenic-intact rats, but did not differ between heated splenic-denervated and nonheated splenic-intact rats. These results support the hypothesis that hyperthermia-induced activation of splenic SND enhances splenic cytokine gene expression. |
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Authors:
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Chanran K Ganta; Frank Blecha; Roman R Ganta; Bryan G Helwig; Sujatha Parimi; Ning Lu; Richard J Fels; Timothy I Musch; Michael J Kenney |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural; Research Support, U.S. Gov't, P.H.S. Date: 2004-08-03 |
Journal Detail:
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Title: Physiological genomics Volume: 19 ISSN: 1531-2267 ISO Abbreviation: Physiol. Genomics Publication Date: 2004 Oct |
Date Detail:
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Created Date: 2004-10-06 Completed Date: 2005-08-18 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9815683 Medline TA: Physiol Genomics Country: United States |
Other Details:
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Languages: eng Pagination: 175-83 Citation Subset: IM |
Affiliation:
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Department of Anatomy and Physiology, Kansas State University, Manhattan, Kansas 66506, USA. |
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Pressure / physiology Computer Systems Cytokines / genetics* Gene Expression Profiling / methods Gene Expression Regulation / physiology* Heart Rate / physiology Hyperthermia, Induced / methods* Rats Rats, Sprague-Dawley Regional Blood Flow / physiology Reverse Transcriptase Polymerase Chain Reaction / methods Spleen / blood supply, chemistry*, innervation, metabolism* Sympathectomy / methods Sympathetic Nervous System / physiology* Synaptic Transmission / physiology |
| Grant Support | |
ID/Acronym/Agency:
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HL-65346/HL/NHLBI NIH HHS; HL-69755/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Cytokines |
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