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Hyperspectral fluorescence imaging for cellular iron mapping in the in vitro model of Parkinson's disease.
MedLine Citation:
PMID:  24297062     Owner:  NLM     Status:  In-Data-Review    
ABSTRACT. Parkinson's disease (PD) is characterized by progressive dopaminergic cell loss in the substantia nigra (SN) and elevated iron levels demonstrated by autopsy. Direct visualization of iron with live imaging techniques has not yet been successful. The aim of this study is to visualize and quantify the distribution of cellular iron using an intrinsic iron hyperspectral fluorescence signal. The 1-methyl-4-phenylpyridinium (MPP+)-induced cellular model of PD was established in SHSY5Y cells exposed to iron with ferric ammonium citrate (FAC, 100 μM). The hyperspectral fluorescence signal of iron was examined using a high-resolution dark-field optical microscope system with signal absorption for the visible/near infrared spectral range. The 6-h group showed heavy cellular iron deposition compared with the 1-h group. The cellular iron was dispersed in a small particulate form, whereas the extracellular iron was aggregated. In addition, iron particles were found to be concentrated on the cell membrane/edge of shrunken cells. The iron accumulation readily occurred in MPP+-induced cells, which is consistent with previous studies demonstrating elevated iron levels in the SN. This direct iron imaging could be applied to analyze the physiological role of iron, and its application might be expanded to various neurological disorders involving metals, such as copper, manganese, or zinc.
Eung Seok Oh; Chaejeong Heo; Ji Seon Kim; Minah Suh; Young Hee Lee; Jong-Min Kim
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of biomedical optics     Volume:  19     ISSN:  1560-2281     ISO Abbreviation:  J Biomed Opt     Publication Date:  2014 May 
Date Detail:
Created Date:  2013-12-03     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9605853     Medline TA:  J Biomed Opt     Country:  United States    
Other Details:
Languages:  eng     Pagination:  51207     Citation Subset:  IM    
Chungnam National University Hospital, Chungnam National University School of Medicine, Department of Neurology, Daejeon, 301-721, Republic of KoreabSeoul National University Bundang Hospital, Department of Neurology, Seongnam, Kyeonggi-do, 463-707, Republic of Korea.
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