Document Detail

Hyperoxia induces the differentiated neuronal phenotype of PC12 cells by producing reactive oxygen species.
MedLine Citation:
PMID:  9425274     Owner:  NLM     Status:  MEDLINE    
Neurite extension of PC12 cells induced by nerve growth factor (NGF) is a well-known model of neuronal differentiation. In this study, the incubation of PC12 cells in a 50% O2 atmosphere (hyperoxia) caused neurite extension. In these cells, amounts of differentiation-marker proteins, tyrosine hydroxylase and neurofilament M increased. The effects of hyperoxia were inhibited by ascorbic acid or N-acetyl-cysteine, antioxidant reagents, suggesting the involvement of reactive oxygen species (ROS). In support of this, artificial generation of free radicals induced the same effects as hyperoxia. In these cells, total phosphorylation of cellular proteins was enhanced similar to NGF-treated cells. These results suggest that hyperoxia enhances the signal for neuronal differentiation by producing ROS, resulting in the induction of the differentiated neuronal phenotype of PC12 cells.
S Katoh; Y Mitsui; K Kitani; T Suzuki
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Biochemical and biophysical research communications     Volume:  241     ISSN:  0006-291X     ISO Abbreviation:  Biochem. Biophys. Res. Commun.     Publication Date:  1997 Dec 
Date Detail:
Created Date:  1998-01-26     Completed Date:  1998-01-26     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372516     Medline TA:  Biochem Biophys Res Commun     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  347-51     Citation Subset:  IM    
Radiation Safety Office, University of Tokyo Hospital, Japan.
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MeSH Terms
Antioxidants / pharmacology
Cell Differentiation / drug effects
Neurons / cytology,  drug effects*
Oxygen / pharmacology*
PC12 Cells
Reactive Oxygen Species / metabolism*
Signal Transduction
Reg. No./Substance:
0/Antioxidants; 0/Reactive Oxygen Species; 7782-44-7/Oxygen

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