Document Detail


Hyperoxia improves oxygen consumption in the detached feline retina.
MedLine Citation:
PMID:  17325181     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
PURPOSE: To investigate the effects of hyperoxia on retinal oxygenation and oxygen consumption in the detached feline retina. METHODS: Retinal detachment was created in nine intact anesthetized cats by injecting 0.25% sodium hyaluronate in balanced salt solution into the subretinal space. Oxygen microelectrodes were used to collect spatial profiles of retinal Po(2) in both the attached and detached retina. A diffusion model was fitted to quantify photoreceptor oxygen consumption (Q(av)). RESULTS: In the detached retina, the Po(2) at the border between the retina and the fluid layer under the retina decreased; hyperoxia increased it to a level that was not significantly different from the control (attached retina, air breathing). Detachment did not change the Po(2) at the border between the avascular and vascularized retina; hyperoxia significantly increased the level. Oxygen consumption decreased to 47% +/- 18% of the control value in the detached retina during normoxia; hyperoxia increased Q(av) to 68% +/- 17% of control. Hyperoxia increased the average inner retinal Po(2) (P(IR)) in the detached retina to a level higher than that during normoxia. Detachment did not change P(IR) during normoxia. CONCLUSIONS: Hyperoxia has been shown to improve photoreceptor survival in the detached retina. The present work suggests that hyperoxia is protective because it allowed increased photoreceptor oxygen consumption. Whereas normal Po(2)s were maintained at the inner and outer border of the avascular region during hyperoxia, Q(av) was not restored to normal, suggesting that other factors are involved in photoreceptor dysfunction during detachment in addition to insufficient oxygen delivery.
Authors:
Shufan Wang; Robert A Linsenmeier
Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural    
Journal Detail:
Title:  Investigative ophthalmology & visual science     Volume:  48     ISSN:  0146-0404     ISO Abbreviation:  Invest. Ophthalmol. Vis. Sci.     Publication Date:  2007 Mar 
Date Detail:
Created Date:  2007-02-27     Completed Date:  2007-04-09     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7703701     Medline TA:  Invest Ophthalmol Vis Sci     Country:  United States    
Other Details:
Languages:  eng     Pagination:  1335-41     Citation Subset:  IM    
Affiliation:
Department of Biomedical Engineering, Northwestern University, Evanston, Illinois, USA.
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MeSH Terms
Descriptor/Qualifier:
Animals
Cats
Cell Survival
Hyperoxia / metabolism*
Ion-Selective Electrodes
Oxygen / metabolism*
Oxygen Consumption / physiology*
Partial Pressure
Photoreceptor Cells, Vertebrate / metabolism
Retina / metabolism*
Retinal Detachment / metabolism*
Grant Support
ID/Acronym/Agency:
R01EY05034/EY/NEI NIH HHS
Chemical
Reg. No./Substance:
7782-44-7/Oxygen

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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