| Hyperoxia and glutathione depletion in the isolated perfused rat liver. | |
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MedLine Citation:
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PMID: 9444885 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Hepatic stores of glutathione may be depleted by hyperoxic exposure or poor nutritional status. We studied the effects of hyperoxia or hepatic glutathione depletion on bile flow rates, and on biliary concentrations of glutathione and amino acids. METHODS: Glutathione depletion was induced in vivo by 1) hyperoxic exposure (O2) for 48 hours, 2) inhibition of glutathione synthesis by treatment with buthionine sulfoximine (BSO), 3) a combination of BSO + O2, or 4) inhibition of cysteine synthesis by propargyglycine (PPG). Livers were then isolated and perfused. RESULTS: Glutathione concentrations in bile, liver, and perfusate were significantly decreased by all treatments. Bile flow was significantly decreased in groups treated with BSO or O2 + BSO, and perfusate LDH was increased by O2 + BSO or PPG. Significant changes in biliary amino acid concentrations included decreased sulfur-containing amino acids and increased branched-chain amino acids in groups treated with BSO, PPG, or O2; and increased essential amino acids in groups treated with O2 or PPG. CONCLUSION: Oxygen exposure or inhibition of glutathione synthesis results in significant decreases in hepatic, perfusate and biliary glutathione concentrations, and increases in biliary amino acids. A decrease in bile flow rate was associated only with the most severe glutathione depletion. |
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Authors:
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K E Shattuck; C D Grinnell; S E Keeney; K Noworyta; D K Rassin |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Journal of investigative medicine : the official publication of the American Federation for Clinical Research Volume: 45 ISSN: 1081-5589 ISO Abbreviation: J. Investig. Med. Publication Date: 1997 Dec |
Date Detail:
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Created Date: 1998-02-12 Completed Date: 1998-02-12 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 9501229 Medline TA: J Investig Med Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 576-83 Citation Subset: IM |
Affiliation:
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Department of Pediatrics, University of Texas Medical Branch, Galveston, TX 77555-0526, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Alkynes
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pharmacology Amino Acids, Sulfur / analysis Animals Bile / drug effects, metabolism, secretion Buthionine Sulfoximine / pharmacology Enzyme Inhibitors / pharmacology Glutathione / metabolism* Glycine / analogs & derivatives, pharmacology Hyperoxia / metabolism* L-Lactate Dehydrogenase / metabolism Liver / drug effects, metabolism* Male Organ Culture Techniques Oxygen / administration & dosage Perfusion Rats Rats, Sprague-Dawley |
| Grant Support | |
ID/Acronym/Agency:
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2SO7-RR05427-30/RR/NCRR NIH HHS; P-30-HD27841/HD/NICHD NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Alkynes; 0/Amino Acids, Sulfur; 0/Enzyme Inhibitors; 5072-26-4/Buthionine Sulfoximine; 56-40-6/Glycine; 64165-64-6/propargylglycine; 70-18-8/Glutathione; 7782-44-7/Oxygen; EC 1.1.1.27/L-Lactate Dehydrogenase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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