Document Detail


Hyperosmotically induced volume change and calcium signaling in intervertebral disk cells: the role of the actin cytoskeleton.
MedLine Citation:
PMID:  12414684     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Loading of the spine alters the osmotic environment in the intervertebral disk (IVD) as interstitial water is expressed from the tissue. Cells from the three zones of the IVD, the anulus fibrosus (AF), transition zone (TZ), and nucleus pulposus (NP), respond to osmotic stress with altered biosynthesis through a pathway that may involve calcium (Ca(2+)) as a second messenger. We examined the hypothesis that IVD cells respond to hyperosmotic stress by increasing the concentration of intracellular calcium ([Ca(2+)](i)) through a mechanism involving F-actin. In response to hyperosmotic stress, control cells from all zones decreased in volume and cells from the AF and TZ exhibited [Ca(2+)](i) transients, while cells from the NP did not. Extracellular Ca(2+) was necessary to initiate [Ca(2+)](i) transients. Stabilization of F-actin with phalloidin prevented the Ca(2+) response in AF and TZ cells and decreased the rate of volume change in cells from all zones, coupled with an increase in the elastic moduli and apparent viscosity. Conversely, actin breakdown with cytochalasin D facilitated Ca(2+) signaling while decreasing the elastic moduli and apparent viscosity for NP cells. These results suggest that hyperosmotic stress induces volume change in IVD cells and may initiate [Ca(2+)](i) transients through an actin-dependent mechanism.
Authors:
Scott Pritchard; Geoffrey R Erickson; Farshid Guilak
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Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Biophysical journal     Volume:  83     ISSN:  0006-3495     ISO Abbreviation:  Biophys. J.     Publication Date:  2002 Nov 
Date Detail:
Created Date:  2002-11-04     Completed Date:  2003-05-19     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0370626     Medline TA:  Biophys J     Country:  United States    
Other Details:
Languages:  eng     Pagination:  2502-10     Citation Subset:  IM    
Affiliation:
Department of Surgery, Duke University Medical Center, 374 Medical Sciences Research Building, Durham, NC 27710, USA.
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MeSH Terms
Descriptor/Qualifier:
Actins / metabolism,  physiology*
Animals
Calcium / metabolism*
Cytoskeleton / metabolism*
Fluorescent Dyes / pharmacology
Intervertebral Disk / metabolism*
Lumbar Vertebrae / metabolism
Microscopy, Confocal
Osmosis*
Osmotic Pressure*
Signal Transduction*
Swine
Time Factors
Grant Support
ID/Acronym/Agency:
AG 15768/AG/NIA NIH HHS; AR 047442/AR/NIAMS NIH HHS; AR 43876/AR/NIAMS NIH HHS; GM 08555/GM/NIGMS NIH HHS
Chemical
Reg. No./Substance:
0/Actins; 0/Fluorescent Dyes; 7440-70-2/Calcium
Comments/Corrections

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